Bacterial Competition Influences the Ability of Symbiotic Bacteria to Colonize Western Flower Thrips.
Bacterial Competition Influences the Ability of Symbiotic Bacteria to Colonize Western Flower Thrips.
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细菌竞争影响共生细菌定殖西花蓟马的能力。
DOI：
10.3389/fmicb.2022.883891
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
共生子介导的RNAi(SMR)是精确控制西花蓟马等害虫的一种很有前途的方法。已知有两种细菌是WFT中的优势共生细菌，即BFo1和BFo2(来自西方Frankliniella ocsidentalis1和2的细菌)，我们在这里通过对获得参考WFT基因组序列的下一代序列数据的分析证实了这一点。我们在WFT中的第一个SMR演示使用了BFo2，它与Pantoea有关，从驯化的荷兰蓟马种群中分离出来。然而，为了成功地利用SMR作为一种蓟马控制措施，这些细菌需要成功地在不同环境中的蓟马种群中定居。在这里，我们描述了一个英国蓟马种群，它没有BFo2，但确实含有BFo1，一种与Erwinia有亲缘关系的物种。试图导入BFo2表明，这种细菌无法在英国蓟马上站稳脚跟，这与表达绿色荧光蛋白的BFo1菌株成功定植形成了鲜明对比。荧光显微镜显示，BFo1在蓟马后中肠和后肠中占据了与BFo2相似的区域。细菌竞争分析表明，BFo2在蓟马上建立的一个障碍是常驻BFo1的身份；从英国蓟马上分离的BFo1比从荷兰蓟马分离的BFo1对BFo2的生长抑制更大，从而允许BFo2的定植。后一株BFo1能够在英国的蓟马上定居，也可能是因为它有能力击败常驻的BFo1。最后，我们观察到，预先暴露于荷兰BFo1的英国蓟马可以成功地被BFo2定殖。这些结果首次表明，微生物竞争和菌株差异可以对共生细菌如何在昆虫物种的不同种群中定居产生很大影响。
Symbiont mediated RNAi (SMR) is a promising method for precision control of pest insect species such as Western Flower Thrips (WFT). Two species of bacteria are known to be dominant symbiotic bacteria in WFT, namely BFo1 and BFo2 (Bacteria from Frankliniella occidentalis 1 and 2), as we here confirm by analysis of next-generation sequence data derived to obtain a reference WFT genome sequence. Our first demonstration of SMR in WFT used BFo2, related to Pantoea, isolated from a domesticated Dutch thrips population. However, for successful use of SMR as a thrips control measure, these bacteria need to successfully colonize different environmental thrips populations. Here, we describe a United Kingdom thrips population that does not harbour BFo2, but does contain BFo1, a species related to Erwinia. Attempts to introduce BFo2 indicate that this bacterium is unable to establish itself in the United Kingdom thrips, in contrast to successful colonization by a strain of BFo1 expressing green fluorescent protein. Fluorescence microscopy indicates that BFo1 occupies similar regions of the thrips posterior midgut and hindgut as BFo2. Bacterial competition assays revealed that a barrier to BFo2 establishing itself in thrips is the identity of the resident BFo1; BFo1 isolated from the United Kingdom thrips suppresses growth of BFo2 to a greater extent than BFo1 from the Dutch thrips that is permissive for BFo2 colonization. The ability of the latter strain of BFo1 to colonize the United Kingdom thrips is also likely attributable to its ability to out-compete the resident BFo1. Lastly, we observed that United Kingdom thrips pre-exposed to the Dutch BFo1 could then be successfully colonized by BFo2. These results indicate, for the first time, that microbial competition and strain differences can have a large influence on how symbiotic bacteria can colonize different populations of an insect species.
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影响因子：
23.8
作者：
Douglas AE
通讯作者：
Douglas AE
影响因子：
3.3
作者：
Facey PD;Méric G;Hitchings MD;Pachebat JA;Hegarty MJ;Chen X;Morgan LV;Hoeppner JE;Whitten MM;Kirk WD;Dyson PJ;Sheppard SK;Del Sol R
通讯作者：
Del Sol R
影响因子：
4
作者：
Beard, CB;Dotson, EM;Durvasula, RV
通讯作者：
Durvasula, RV
影响因子：
3.4
作者：
Chanbusarakum, Lisa;Ullman, Diane
通讯作者：
Ullman, Diane
DOI：
10.1098/rspb.2008.1476
发表时间：
2009-03-07
影响因子：
4.7
作者：
Guenduez, E. Akman;Douglas, A. E.
通讯作者：
Douglas, A. E.