Differential expression of circRNAs during rat lung development
Differential expression of circRNAs during rat lung development
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DOI：
10.3892/ijmm.2019.4299
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发表时间：
2019-10-01
影响因子：
5.4
通讯作者：
Yang, Yang
中科院分区：
文献类型：
--
作者：
Shen, Yan-Qing;Pan, Jing-Jing;Yang, Yang
目前，在癌症和不同物种的各种组织中已经发现了数以千计的环状RNA。然而，CircRNAs在大鼠肺发育过程中的表达在很大程度上仍不清楚。本研究采用CircRNA高通量测序技术，对大鼠胚胎发育过程中3个时间点[胚胎(E)19、E21和出生后(P)第3天]的三种混合肺组织的CircRNA表达谱进行了筛选。在4个时间点(E16、E19、E21和P3)用逆转录-聚合酶链式反应(RT-PCR)验证初步结果。在E19、E21和P3的3个时间点(E19、E21和P3)之间，共有375个CircRNA在E19和E21中有不同的表达(倍数变化和Gt；=1.5；P=1.5；P=1.5)，并在4个时间点(E16、E19、E21和P3)进行RT-PCR。随后，基因本体论和京都百科全书对差异表达的CircRNAs的亲本基因进行了分析，发现这些CircRNAs可能在肺发育中发挥重要作用。本研究结果支持这些新发现的CircRNA参与肺发育。此外，这些发现可能有助于阐明正常大鼠肺发育的生理病理机制，并可能进一步为肺发育性疾病提供生理学基础。
At present, thousands of circular RNAs (circRNAs) have been found in cancer and various tissues from different species. However, the expression of circRNAs during rat lung development remains largely unknown. In the present study, circRNA expression profiles were screened in three mixed rat lung tissues at 3 time-points [embryonic day (E) 19, E21 and post-natal (P) day 3] during fetal rat development with circRNA high-throughput sequencing. Preliminary results were verified by reverse transcription-PCR (RT-PCR) at 4 time-points (E16, E19, E21 and P3). A total of 375 circRNAs were differently expressed in E19 vs. E21 (fold change >= 1.5; P= 1.5; P= 1.5) between 3 time-points (E19, E21 and P3) and were selected for RT-PCR at 4 time-points (E16, E19, E21 and P3). Subsequently, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of parent genes of the differentially expressed circRNAs revealed that these circRNAs may serve important roles in lung development. The present results support that these new found circRNAs participate in lung development. Furthermore, these findings may help to clarify the physiopathological mechanism of normal rat lung development, and may further provide a physiopatho-logical basis of lung developmental diseases.