Histone H3 lysine 9 methyltransferase FvDim5 regulates fungal development, pathogenicity and osmotic stress responses in Fusarium verticillioides
Histone H3 lysine 9 methyltransferase FvDim5 regulates fungal development, pathogenicity and osmotic stress responses in Fusarium verticillioides
复制标题
组蛋白 H3 赖氨酸 9 甲基转移酶 FvDim5 调节轮枝镰刀菌的真菌发育、致病性和渗透应激反应
DOI：
10.1093/femsle/fnx184
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发表时间：
2017-10-01
影响因子：
2.1
通讯作者：
Gao, Xuewen
中科院分区：
文献类型：
--
作者：
Gu, Qin;Ji, Tiantian;Gao, Xuewen
组蛋白甲基化在真核细胞中起着重要的生物学作用。组蛋白H3 （H3K9me）上赖氨酸9的甲基化对调节染色质结构和基因转录至关重要。Dim5是一种赖氨酸组蛋白甲基转移酶（KHMTase），在真核生物中负责H3K9的甲基化。在本研究中，我们在黄萎病镰刀菌中鉴定了一个单一的粗神经孢子菌Dim5的同源物。在本研究中，我们报道FvDim5调控H3K9的三甲基化（H3K9me3）。FvDIM5缺失突变体（ΔFvDim5）在分生、表皮生成和真菌毒力方面存在显著缺陷。出乎意料的是，我们发现FvDIM5的缺失导致对渗透胁迫的耐受性增加和FvHog1磷酸化上调。这些结果表明FvDim5在黄萎病真菌发育、致病性和渗透胁迫反应的调控中具有重要作用。
Histone methylation plays important biological roles in eukaryotic cells. Methylation of lysine 9 at histone H3 (H3K9me) is critical for regulating chromatin structure and gene transcription. Dim5 is a lysine histone methyltransferase (KHMTase) enzyme, which is responsible for the methylation of H3K9 in eukaryotes. In the current study, we identified a single ortholog of Neurospora crassa Dim5 in Fusarium verticillioides. In this study, we report that FvDim5 regulates the trimethylation of H3K9 (H3K9me3). The FvDIM5 deletion mutant (ΔFvDim5) showed significant defects in conidiation, perithecium production and fungal virulence. Unexpectedly, we found that deletion of FvDIM5 resulted in increased tolerance to osmotic stresses and upregulated FvHog1 phosphorylation. These results indicate the importance of FvDim5 for the regulation of fungal development, pathogenicity and osmotic stress responses in F. verticillioides.