CHARACTERIZATION OF A YEAST REPLICATION ORIGIN (ARS2) AND CONSTRUCTION OF STABLE MINICHROMOSOMES CONTAINING CLONED YEAST CENTROMERE DNA (CEN3)
CHARACTERIZATION OF A YEAST REPLICATION ORIGIN (ARS2) AND CONSTRUCTION OF STABLE MINICHROMOSOMES CONTAINING CLONED YEAST CENTROMERE DNA (CEN3)
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DOI：
10.1016/0378-1119(81)90125-6
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
CARBON, J
中科院分区：
文献类型：
--
作者：
HSIAO, CL;CARBON, J
A yeast DNA sequence (ars2), capable of supporting autonomous replication of plasmids in yeast, is described. The ars2 replicator occurs .apprx. 7 kb [kilobase] from the ARG4 gene on yeast chromosome VIII. Plasmids containing ars2 and the ARG4 gene transform yeast arg4 mutants to ARG4+ with high frequency (.apprx. 103 transformants/.mu.g DNA) and replicate autonomously in the transformed cells. The ars2 plasmids are mitotically unstable and are readily lost from yeast cultures when grown under nonselective conditions. The addition of a DNA segment containing a functional yeast centromere (CEN3) and an ars2 plasmid effectively stabilizes the plasmid against both mitotic and meiotic loss. The ars2-CEN3 minichromosomes replicate autonomously in controlled copy number while segregating in a typical Mendelian pattern (2+ :2-)during meiosis. The requirement for a separate replicator sequence for stable mitotic and meiotic maintenance of centromere-containing minichromosomes is equally satisfied by the presence of either ars1 or ars2. The centromere controls plasmid copy number to a low value (usually 1) regardless of the type of replicator used.
