Comparison between the efficiencies of columns packed with fully and partially porous C18-bonded silica materials
Comparison between the efficiencies of columns packed with fully and partially porous C18-bonded silica materials
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DOI：
10.1016/j.chroma.2007.05.030
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发表时间：
2007-07-20
影响因子：
4.1
通讯作者：
Guiochon, Georges
中科院分区：
文献类型：
--
作者：
Gritti, Fabrice;Cavazzini, Alberto;Guiochon, Georges
将新品牌的壳颗粒的色谱性能与具有相似尺寸的常规品牌的完全多孔二氧化硅颗粒的色谱性能进行比较。新材料（Halo，Advanced Materials Technology，威尔明顿，DE）由2.7 μ m颗粒制成，所述颗粒存在于被0.5 μ m厚的多孔二氧化硅壳覆盖的1.7 μ m固体核中。另一种材料由常规的3 μ m商业二氧化硅-B材料的多孔颗粒组成。这两个列具有相同的尺寸，150转x 4.6毫米。减少板高度的两个低分子量化合物，萘和蒽，两种肽（赖氨酸-缓激肽和缓激肽），和四种蛋白质，胰岛素，溶菌酶，P-乳球蛋白，和牛血清白蛋白进行了测量，在一个宽的流速范围内，并分析的基础上的货车Deemter方程和现代模型的条款。Halo柱提供了比其它柱更小的轴向扩散系数B和更小的涡流扩散项A，这一结果与其更低的内部孔隙度（是（P）= 0的元素）一致。19对0.42）并且具有较窄的颗粒尺寸分布（σ = 5%对13%）。对于两种低分子量化合物和两种肽，两个色谱柱具有相似的C项。然而，未被排除的蛋白质的C项在填充有Halo颗粒的柱上明显低于其他柱。最近对壳颗粒中传质动力学的理论分析预测了适度保留蛋白质的C tenn（3 < k' < 5），壳比完全多孔颗粒低约35%，而实验数据显示该值低近45%，考虑到颗粒的内部曲折度可能不同，这是一个极好的一致性，影响两种材料中蛋白质的有效扩散率（D-eff）的比率。令人惊讶的是，Halo填充柱的Kozeny-Carman常数比另一柱的Kozeny-Carman常数大50%，尽管Halo柱的渗透率略大。由于其较大的外部孔隙率。(c)2007年由Elsevier B. V.出版。
The chromatographic performance of a new brand of shell particles is compared to that of a conventional brand of totally porous silica particles having a similar size. The new material (Halo, Advanced Materials Technology, Wilmington, DE) is made of 2.7 VLm particles that consist in a 1.7 mu m solid core covered with a 0.5 mu m thick shell of porous silica. The other material consists of the porous particles of a conventional 3 mu m commercial silica-B material. These two columns have the same dimensions, 150 turn x 4.6 mm. The reduced plate heights of two low molecular weight compounds, naphthalene and anthracene, two peptides (lys-bradykinin and bradykinin), and four proteins, insulin, lysozyme, P-lactoglobulin, and bovine serum albumin were measured in a wide flow rate range and analyzed on the basis of the Van Deemter equation and of modem models for its terms. The Halo column provides a smaller axial diffusion coefficient B and a smaller eddy dispersion term A than the other column, a result consistent with its lower internal porosity (is an element of(P) = 0. 19 versus 0.42) and with the narrower size distribution of its particles (sigma = 5% versus 13%). The two columns have similar C terms for the two low molecular weight compounds and for the two peptides. However, the C term of the proteins that are not excluded is markedly lower on the column packed with the Halo particles than on the other column. A recent theoretical analysis of the mass transfer kinetics in shell particles predicts a C tenn for moderately retained proteins (3 < k' < 5) that is about 35% lower for shell than for fully porous particles while the experimental data show a value nearly 45% lower, an excellent agreement considering that the internal tortuosity of the particles might be different, affecting the ratio of the effective diffusivities (D-eff) of the proteins in the two materials. Surprisingly, the Kozeny-Carman constant of the Halo packed column is 50% larger than that of the other column, in spite of which the permeability of the Halo column is slightly larger. due to its larger external porosity. (c) 2007 Published by Elsevier B.V.