Selected Reaction Monitoring Applied to Quantitative Proteomics
Selected Reaction Monitoring Applied to Quantitative Proteomics
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DOI：
10.1007/978-1-60761-780-8_9
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发表时间：
2010-01-01
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影响因子：
--
通讯作者：
Domon, Bruno
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文献类型：
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作者：
Kiyonami, Reiko;Domon, Bruno
蛋白质组学正逐渐从纯粹的定性研究（蛋白质鉴定）转向大规模的定量实验，这是由于越来越需要对生物样品中的大量蛋白质进行一致和精确的分析。选择性反应监测（SRM）技术由于其选择性（两级质量选择）、灵敏度（非扫描模式）和宽动态范围等优点，越来越多地应用于定量蛋白质组学。本文描述了SRM实验的设计和实验设置的不同步骤。
Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.