A HIGH-FREQUENCY OF HUMAN PAPILLOMAVIRUS DNA-SEQUENCES IN CERVICAL CARCINOMAS OF INDIAN WOMEN AS REVEALED BY SOUTHERN BLOT HYBRIDIZATION AND POLYMERASE CHAIN-REACTION
A HIGH-FREQUENCY OF HUMAN PAPILLOMAVIRUS DNA-SEQUENCES IN CERVICAL CARCINOMAS OF INDIAN WOMEN AS REVEALED BY SOUTHERN BLOT HYBRIDIZATION AND POLYMERASE CHAIN-REACTION
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DOI：
10.1002/jmv.1890360402
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发表时间：
1992-04-01
影响因子：
12.7
通讯作者：
LUTHRA, UK
中科院分区：
文献类型：
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作者：
DAS, BC;SHARMA, JK;LUTHRA, UK
采用Southern印迹杂交和聚合酶链反应（PCR）检测了96例宫颈鳞状上皮癌阴道镜活检标本和22例年龄匹配的正常对照活检标本中是否存在不同的人乳头瘤病毒（HPV）DNA类型。子宫颈癌是印度妇女最常见的恶性疾病，与世界其他地区报告的情况相比，HPV的发生率很高（98%）。发现HPV 16型是主要类型（64%），而HPV 18型的频率很低（3%）。在HPV的个体分型中，除了一例HPV 11型外，在严格的杂交条件下，没有发现活检包含任何其他已知的HPV类型。只有1例HPV 16型和18型双重感染。在低严格性条件下的杂交与HPV 16型和18型的混合探针，29个额外的活检被发现是阳性的。单独Southern印迹杂交在92%的病例中检测到HPV DNA，但对照组中没有。PCR检测HPV阳性者6例（6.25%），健康妇女4例（18.18%）。对HPV 16的物理状态的分析表明，约70%的癌病例中存在整合，而其中30%为游离型。研究结果表明，HPV感染是宫颈癌发展的重要病因因素，许多此类肿瘤可能在没有HPV感染的情况下出现，并且病毒DNA整合到宿主基因组中并不总是恶性进展所必需的。此外，PCR的使用为HPV感染的有意义的流行病学研究提供了Southern印迹杂交的有效补充。
Ninety-six colposcopically directed biopsies from squamous epithelial carcinoma of the uterine cervix and 22 age-matched normal control biopsy specimens were examined by both Southern blot hybridization and polymerase chain reaction (PCR) for the presence of different human papillomavirus (HPV) DNA types. Cancer of the uterine cervix, which is the most common malignant disease in Indian women, showed a high frequency (98%) of HPV as compared to those reported from other parts of the world. HPV type 16 was found to be the dominant (64%) type while the frequency of HPV type 18 was very low (3%). On individual typing of HPV, no biopsy was found to contain any other known HPV types under stringent conditions of hybridization except a single case of HPV type 11. Only one case of double infection with HPV types 16 and 18 was recorded. Under low stringency conditions of hybridization with a mixed probe of HPV types 16 and 18, 29 additional biopsies were found to be positive. Southern blot hybridization alone detected HPV DNA in 92% of the cases but none in the controls. By PCR, six (6.25%) more cases and four (18.18%) healthy women were found to be positive for HPVs. Analysis of the physical state of HPV 16 indicated integration in about 70% of carcinoma cases while 30% of them were in episomal form. The findings suggest that infection with HPV is an important etiologic factor for the development of cervical cancer, that a number of such tumours may arise without HPV infection, and that integration of the viral DNA into host genome is not always essential for malignant progression. Furthermore, the use of PCR provides an effective complementation of Southern blot hybridization for meaningful epidemiological studies of HPV infection.