Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus
Mucosal and systemic isotype-specific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus
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DOI：
10.1016/s0165-2427(01)00417-2
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发表时间：
2002-02-01
影响因子：
1.8
通讯作者：
Rubio, P
中科院分区：
文献类型：
--
作者：
de Arriba, ML;Carvajal, A;Rubio, P
用两种不同剂量的细胞培养菌株CV-777的猪腹泻病毒（PEDV）或相同菌株的毒性分离物和相同的毒品pedv 3挑战，将11天的常规饲养小猪与两种不同剂量的细胞培养菌株CV-777接种口服。几周后。接种了两剂衰减病毒的猪没有表现出任何典型的疾病迹象，并且病毒脱落并不常见。相比之下，在100％的100％中证明了暴露于毒力pedv的猪中31％。在不同的通讯日（PID）和挑战后日（PCD）病毒特异性抗体分泌细胞（ASC）（ASC）（肠道相关淋巴组织（Duododenum和Duododenum和ileum lamina propria and Propric lamina and肠系膜淋巴结））和全身性位置（Blood and Spleen）（血液）酶联免疫疗程（ELISPOT）。在接种衰减的PEDV的组中，仅检测到很小的反应，而在PID 21上暴露于毒性病毒的组中，检测到大量IgG和IgA ASC。 ELISA研究了血清中同种型特异性抗体反应。尽管最高反应对应于接种毒性病毒的组，但只有该组显示IgA反应。暴露于有毒佩德维夫的猪被PID 21上的较高剂量的同一毒病毒完全保护了挑战，并且没有脱离病毒。接种衰减菌株的猪受到部分保护，以防止挑战，而低剂量的低剂量和50％的高剂量暴露的猪在挑战后不会脱落病毒。对照组的所有猪，以前未暴露于病毒，都在粪便中排出病毒。在挑战日，在肠道相关的淋巴组织和血液中检测到的ASC反应之间以及当天的保护与血清同种型特异性抗体滴度之间的ASC反应之间建立了强大的正相关。此外，PID 21的血液中检测到的IgA和IgG ASC反应也与肠道相关淋巴组织中发现的反应有关。挑战后的ASC和血清抗体反应对应于接种衰减病毒的组中的二次免疫反应，而对照组中显而易见的主要反应。在接种有毒的PEDV接种的猪中研究的任何参数中均未发现增加。 （c）2002 Elsevier Science B.V保留所有权利。
Eleven-day-old conventionally reared piglets were inoculated orally with two different doses of the cell-culture adapted strain CV-777 of the porcine epidemic diarrhoea virus (PEDV) or the virulent isolate of the same strain and challenged with the same virulent PEDV 3 weeks later. Pigs inoculated with the two doses of the attenuated virus did not show any typical sign of the disease, and virus shedding was not frequent. In contrast, 31% of pigs exposed to the virulent PEDV developed diarrhoea and virus shedding was demonstrated in 100%. At different postinoculation day (PID) and postchallenge day (PCD) virus-specific antibody-secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (blood and spleen) were assessed by enzyme-linked immunospot (ELISPOT). Only a small response was detected in the groups inoculated with attenuated PEDV, whereas in the group previously exposed to the virulent virus on PID 21 a large number of IgG and IgA ASC was detected. Isotype-specific antibody responses in serum were investigated by ELISA. IgG responses were detected in all groups, although the highest response corresponded to the group inoculated with virulent virus and only this group showed an IgA response. The pigs exposed to virulent PEDV were completely protected against the challenge with a higher dose of the same virulent virus on PID 21 and none of them shed the virus. The pigs inoculated with the attenuated strain were partially protected against the challenge, and 25% of the low dose- and 50% of the high dose-exposed pigs did not shed virus after challenge. All the pigs from a control group, not previously exposed to the virus, excreted the virus in faeces. A strong positive correlation was established between protection and the ASC responses detected in gut associated lymphoid tissues and blood at the challenge day and also between protection and serum isotype-specific antibody titers on that day. In addition, the IgA and IgG ASC responses detected in the blood on PID 21 also correlated with the responses found in the gut associated lymphoid tissues. The ASC and serum antibody responses after the challenge corresponded to a secondary immune response in the groups inoculated with attenuated virus, whereas a primary response was evident in the control group. No increase was seen in any of the parameters studied in the pigs inoculated with virulent PEDV. (C) 2002 Elsevier Science B.V All rights reserved.