[Effects of cordyceps sinensi on bleomycin-induced pulmonary fibrosis in mice].
[Effects of cordyceps sinensi on bleomycin-induced pulmonary fibrosis in mice].
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发表时间：
2007-12
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通讯作者：
Shao-jie Wang;Wen Bai;Chun-ling Wang;Zhong Dai
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作者：
Shao-jie Wang;Wen Bai;Chun-ling Wang;Zhong Dai
目的研究冬虫夏草对博莱霉素诱导的小鼠肺纤维化的防治作用。方法大鼠分为4组。BM组（模型组）通过鼻腔滴注博莱霉素5 mg × kg（-1）建立肺间质纤维化模型。PCT组（冬虫夏草防治组）在滴注博莱霉素前48 h口服冬虫夏草。CT组（紫堇治疗组）于博莱霉素滴注后14 d灌胃紫堇，NC组（正常对照组）灌胃紫堇。28 d时收集支气管肺泡灌洗液（BALF）和肺组织，右肺行HE染色和Masson染色，电镜下观察细胞显微结构的变化，酸解法检测左肺组织羟脯氨酸（HYP）含量，ELISA法检测BALF中TGF-β 1、IFN-γ和IL-4的含量。结果与BM组相比，PCT组和CT组小鼠肺系数显著降低（P < 0.05），肺泡炎和肺纤维化程度明显减轻（P < 0.05），肺组织HYP含量降低（P < 0.05）。各药物干预组BALF中TGF-β 1和IL-4含量均显著降低，IFN-γ含量显著升高（P < 0.05）。结论冬虫夏草能在一定程度上减轻博莱霉素所致肺泡炎及纤维化程度。其作用机制可能与抑制肺组织TGF-β 1的表达及调节I、II型细胞因子的失衡有关。
OBJECTIVE To study the prevention & treatment effect of cordyceps sinensi on bleomycin-induced pulmonary fibrosis (PF) in mice. METHOD Rats were divided into 4 groups. In BM group (model), pulmonary interstitial fibrosis model was established through nasal dripping of 5 mg x kg(-1) bleomycin. In PCT group (prevention & treatment with cordyceps sinensi), cordyceps sinensi was orally administered 48 h before bleomycin dripping. In CT group (treatment with cordyceps sinensi), cordyceps sinensi was orally administered at 14 d after bleomycin dripping, and NC group (normal mice, control). At 28 d, bronchoalveolar lavage fluid (BALF) and lung tissue were collected, right lung received HE staining and masson staining, the change in cell micro-structure was observed under electron microscope, the hydroxyproline (HYP) content in lung tissue of left lung was detected through acidolysis method, and TGF-beta1, IFN-gamma and IL-4 concentration in BALF was detected through ELISA. RESULT Compared with those in BM group, mouse lung coefficient decreased significantly (P < 0.05), alveolitis and fibrosis degree improved significantly in PCT and CT groups (P < 0.05), HYP content in lung decreased (P < 0.05). TGF-beta1 and 1L-4 content in BALF decreased significantly in each drug intervention group, IFN-gamma content increased significantly (P < 0.05). CONCLUSION The cordyceps sinensi can alleviate bleomycin-induced alveolitis and fibrosis degree in a certain degree. The mechanism of the alleviation is possibly due to its inhibition of TGF-beta1 expression and its regulation of imbalance of the type I and II cytokine in lung tissue.