Interferon-sensitive mutants of mengovirus. I. Isolation and biological characterization.
Interferon-sensitive mutants of mengovirus. I. Isolation and biological characterization.
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孟戈病毒的干扰素敏感突变体。
DOI：
10.1016/0042-6822(76)90501-8
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发表时间：
1976
期刊：
影响因子：
3.7
通讯作者：
P. Brandman
中科院分区：
文献类型：
--
作者：
E. H. Simon;S. Kung;T. Koh;P. Brandman
已分离出三种对干扰素敏感的孟戈病毒突变体，并对其中一种（is-1）的特性进行了详细研究。该突变体is-1在对照L细胞中正常生长，但在用干扰素预处理的细胞中，其产量比野生型（is+）低至少10倍。此外，IS-1达到其最终产量的95%，无论是在存在或不存在干扰素的感染后12小时，而在受保护的细胞，IS+不达到这个水平，直到20小时感染后。在干扰素存在的混合感染中，is+可以拯救is-1，这意味着突变影响扩散产物。用10个单位的干扰素保护并用this-1感染的大多数细胞在感染后存活，并且能够形成克隆，如通过集落形成能力所测量的。如果受保护的细胞在感染后立即用放线菌素D处理，则来自被this-1感染的细胞的产量提高，而来自被this+感染的细胞的产量降低，最终结果是完全逆转了this-1表型。这些结果与theis-1功能保护病毒基因组不受诱导的细胞核酸酶影响的假设一致。
Three interferon-sensitive (is) mutants of mengovirus have been isolated and the properties of one (is-1) studied in detail. This mutant,is-1, grows normally in control L cells, but in cells pretreated with interferon, its yield is at least 10-fold lower than wild type (is+). Furthermore,is-1 attains 95% of its final yield 12 hr postinfection whether in the presence or absence of interferon, whereas in protected cells,is+does not reach this level until 20 hr postinfection. In mixed infection in the presence of interferon,is+can rescueis-1, implying that the mutation affects a diffusible product. Most cells protected with 10 units of interferon and infected withis-1 survive the infection and are able to form clones, as measured by colony-forming ability. If protected cells are treated with actinomycin D immediately following infection, the yields from cells infected withis-1 are enhanced and the yields from cells infected withis+are lowered, the net result being complete reversal of theis-1 phenotype. These results are consistent with the hypothesis that theis-1 function protects the virus genome from an induced cellular nuclease.