Identification and characterization by electrospray mass spectrometry of endogenous Drosophila sphingadienes
Identification and characterization by electrospray mass spectrometry of endogenous Drosophila sphingadienes
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DOI：
10.1194/jlr.m700414-jlr200
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发表时间：
2008-03-01
影响因子：
6.5
通讯作者：
Saba, Julie D.
中科院分区：
文献类型：
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作者：
Fyrst, Henrik;Zhang, Xinyi;Saba, Julie D.
鞘磷脂由一组复杂的脂类组成，这些脂类集中在膜筏中，其代谢物起信号分子的作用。鞘磷脂在果蝇中是保守的，在果蝇中，它们的严格调控是正常发育和组织完整性所必需的。在这项研究中，我们鉴定了一个新的果蝇鞘脂家族，该家族在长链碱基(LCB)上含有两个双键。野生型果蝇体内脂类含量较低，而不表达鞘氨醇-L-磷酸裂解酶、鞘磷脂分解缺陷的果蝇突变体中脂类显著积聚。为了确定未知脂类的身份，在C18-高效液相色谱柱上分离纯化的苍蝇全脂提取物，并用电喷雾质谱仪进行分析。这些脂肪含有14或16个碳的LCB，在C4，6上有共轭双键。A(4，6)-鞘氨二烯被发现为游离LCB，磷酸化LCB，以及神经酰胺中的狮身人面像碱基。
Sphingolipids comprise a complex group of lipids concentrated in membrane rafts and whose metabolites function as signaling molecules. Sphingolipids are conserved in Drosophila, in which their tight regulation is required for proper development and tissue integrity. In this study, we identified a new family of Drosophila sphingolipids containing two double bonds in the long chain base (LCB). The lipids were found at low levels in wild-type flies and accumulated markedly in Drosophila Sply mutants, which do not express sphingosine-l-phosphate lyase and are defective in sphingolipid catabolism. To determine the identity of the unknown lipids, purified whole fly lipid extracts were separated on a C18-HPLC column and analyzed using electrospray mass spectrometry. The lipids contain a LCB of either 14 or 16 carbons with conjugated double bonds at C4,6. The A(4,6)-sphingadienes were found as free LCBs, as phosphorylated LCBs, and as the sphingoid base in ceramides.jr The temporal and spatial accumulation of A(4,6)-sphingadienes in Sply mutants suggests that these lipids may contribute to the muscle degeneration observed in these flies.