Nuclear export of MAP kinase (ERK) involves a MAP kinase kinase (MEK)-dependent active transport mechanism.
Nuclear export of MAP kinase (ERK) involves a MAP kinase kinase (MEK)-dependent active transport mechanism.
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DOI：
10.1083/jcb.148.5.849
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发表时间：
2000-03-06
期刊：
影响因子：
--
通讯作者：
Nishida E
中科院分区：
文献类型：
--
作者：
Adachi M;Fukuda M;Nishida E
响应于细胞外刺激，在静止细胞中定位于细胞质的丝裂原活化蛋白激酶（MAPK，也称为ERK）易位到细胞核，然后再次重新定位到细胞质。放线菌酮不能抑制核MAPK向细胞质的重新定位，证实了重新定位是通过核输出而不是合成MAPK实现的。MAPK的核输出被一种核输出信号（内斯）依赖性转运的特异性抑制剂来普霉素B（LMB）所抑制。然后，我们已经表明，MAP激酶激酶（MAPKK，也称为MEK），它主要定位于细胞质，因为它具有内斯，能够不断穿梭于细胞质和细胞核之间。MAPK，当注射到细胞核中，迅速出口从细胞核共注射野生型MAPKK，但不是由NES破坏的MAPKK。此外，将与MAPK结合位点相对应的片段注射到细胞核中，这将破坏MAPK与MAPK在细胞核中的结合，从而显著抑制内源性MAPK的核输出。两者合计，这些结果表明，重新定位的核MAPK的细胞质涉及MAPK依赖的，主动运输机制。
In response to extracellular stimuli, mitogen-activated protein kinase (MAPK, also known as ERK), which localizes to the cytoplasm in quiescent cells, translocates to the nucleus and then relocalizes to the cytoplasm again. The relocalization of nuclear MAPK to the cytoplasm was not inhibited by cycloheximide, confirming that the relocalization is achieved by nuclear export, but not synthesis, of MAPK. The nuclear export of MAPK was inhibited by leptomycin B (LMB), a specific inhibitor of the nuclear export signal (NES)-dependent transport. We have then shown that MAP kinase kinase (MAPKK, also known as MEK), which mostly localizes to the cytoplasm because of its having NES, is able to shuttle between the cytoplasm and the nucleus constantly. MAPK, when injected into the nucleus, was rapidly exported from the nucleus by coinjected wild-type MAPKK, but not by the NES-disrupted MAPKK. In addition, injection of the fragment corresponding to the MAPK-binding site of MAPKK into the nucleus, which would disrupt the binding of MAPK to MAPKK in the nucleus, significantly inhibited the nuclear export of endogenous MAPK. Taken together, these results suggest that the relocalization of nuclear MAPK to the cytoplasm involves a MAPKK-dependent, active transport mechanism.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
11.4
作者：
Fukuda, M;Gotoh, Y;Nishida, E
通讯作者：
Nishida, E
影响因子：
7.5
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
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DOI：
10.1073/pnas.94.8.3742
发表时间：
1997-04-15
影响因子：
11.1
作者：
Jaaro, H;Rubinfeld, H;Seger, R
通讯作者：
Seger, R