Differential attachment of human neoplastic B cells to purified extracellular matrix molecules.
Differential attachment of human neoplastic B cells to purified extracellular matrix molecules.
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人类肿瘤 B 细胞与纯化的细胞外基质分子的差异附着。
作者：
D. Segat;C. Pucillo;G. Marotta;R. Perris;A. Colombatti
正常和肿瘤淋巴细胞的再循环是通过与内皮细胞表面结合，然后渗出，随后细胞与基底膜和基质细胞外基质(ECM)的成分相互作用而发生的。为了确定可能参与肿瘤B细胞组织扩散的基质成分，我们检测了三个淋巴瘤B细胞系和一个费城染色体(PH1)阳性细胞系的能力，这些细胞系是从慢性髓系白血病(CML)的淋巴转化建立的，能够附着一系列纯化的ECM分子。用一组标记物进行免疫表型鉴定表明，这些细胞系来自于在B细胞成熟的不同阶段经历了转化的细胞。这四种细胞系显示出不同的能力，可以附着在测试的细胞外基质分子上。BV-173、CI-1和SC-1细胞与纤维连接蛋白(FN)有不同程度的黏附。RI-1、CI-1和SC-1细胞与人层粘连蛋白(LN)变异体结合，而只有CI-1和SC-1细胞对VI型胶原(Col)表现出一定的亲和力。4种细胞株均与I型胶原相互作用，但只有BV-173和Ri-1细胞与III型纤维粘连蛋白结合。在所有病例中，细胞在组装成聚合纤维时与纤维状胶原结合，而不能附着于单体和变性的胶原。与细胞对FN和LN的粘附性不同，细胞对FN和LN的粘附性在高底物浓度时表现出平台性，而对I型胶原纤维的粘附性在中等浓度时达到峰值，然后下降，这表明细胞对胶原纤维的特定大分子排列有反应。与单个ECM分子的黏附与细胞的表观成熟状态或已知ECM受体的相对密度没有直接关系。综上所述，这些结果表明，肿瘤B细胞与选定的基质成分的相互作用可能会影响它们在组织中的扩散。我们进一步认为，在体外使用定量细胞黏附试验可能提供在体内确定肿瘤B细胞行为特征的手段。
Recirculation of normal and neoplastic lymphocytes occurs via binding to the endothelial luminar surface, followed by extravasation and the subsequent interaction of the cells with components of the underlying basement membrane and stromal extracellular matrix (ECM). To identify matrix constituents that could be involved in the tissue dissemination of neoplastic B cells, we have examined the ability of three lymphoma B-cell lines and one Philadelphia chromosome (Ph1)-positive cell line established from the lymphoid transformation of a chronic myeloid leukemia (CML) to adhere to a range of purified ECM molecules. Immunophenotyping with a panel of markers suggested that the lines derived from cells that had undergone transformation at distinct stages of B-cell maturation. The four cell lines displayed a differential ability to adhere to the ECM molecules tested. BV-173, Ci-1, and Sc-1 cells attached to various degrees to fibronectin (FN). Ri-1, Ci-1, and Sc-1 cells attached to human laminin (LN) variants, whereas only Ci-1 and Sc-1 cells showed some affinity for collagen (Col) type VI. All four cell lines interacted with fibrillar Col I, but only BV-173 and Ri-1 cells attached to fibrillar Col III. The subendothelial Col VIII only was active as a substratum for BV-173 cells. In all cases, cells bound to fibrillar collagens when they were assembled into polymeric fibrils, and were incapable of adhering to monomeric and denatured collagen. In contrast to cell adhesion to FN and LN, which showed a plateau at high substrate concentrations, adhesion to fibrillar Col I reached a peak at intermediary concentrations and decreased thereafter, suggesting that cells respond to a definite macromolecular arrangement of collagenous fibrils. Adhesion to individual ECM molecules was not directly correlated with the apparent maturation state of the cells, nor with the relative density of known ECM receptors. Taken together, these results suggest that interaction of neoplastic B cells with selected matrix components may influence their dispersion throughout tissues. We further suggest that the use of quantitative cell adhesion assays in vitro may provide means of defining the behavioral traits of neoplastic B cells in vivo.
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影响因子：
4
作者：
R. Perris;J. Syfrig;M. Paulsson;M. Bronner‐Fraser
通讯作者：
R. Perris;J. Syfrig;M. Paulsson;M. Bronner‐Fraser
人类 B 细胞前体与骨髓微环境的成熟依赖性粘附。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ryan,DH;Nuccie,BL;Abboud,CN;Liesveld,JL
通讯作者：
Liesveld,JL
通过有限的蛋白水解从胎盘中分离出几乎完整的、具有生物活性的人层粘连蛋白。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wewer,U;Albrechtsen,R;Manthorpe,M;Varon,S;Engvall,E;Ruoslahti,E
通讯作者：
Ruoslahti,E
DOI：
10.1073/pnas.87.9.3264
发表时间：
1990-05-01
影响因子：
11.1
作者：
EHRIG, K;LEIVO, I;ENGVALL, E
通讯作者：
ENGVALL, E
影响因子：
20.3
作者：
Uchiyama,H;Barut,BA;Chauhan,D;Cannistra,SA;Anderson,KC
通讯作者：
Anderson,KC