New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt's Lymphoma Cells.
New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt's Lymphoma Cells.
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DOI：
10.3390/cancers10010012
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发表时间：
2018-01-05
期刊：
影响因子：
5.2
通讯作者：
Wiels J
中科院分区：
文献类型：
--
作者：
Chelouah S;Cochet E;Couvé S;Balkaran S;Robert A;May E;Ogryzko V;Wiels J
EB病毒核抗原前导蛋白（EBNA-LP）作为EBNA-2的共激活物，EBNA-2是EB病毒（EBV）诱导的B细胞转化所必需的转录激活物。伯基特淋巴瘤（BL）细胞携带突变型EB病毒株，该株缺乏EBNA-2基因和EBNA-LP的3 '外显子，表达Y1 Y2截短的EBNA-LP亚型（tEBNA-LP），并且比感染野生型病毒时更好地抵抗细胞凋亡。在这样的BL细胞中，tEBNA-LP与蛋白磷酸酶2A（PP 2A）催化亚基（PP 2A C）相互作用，并且这种相互作用可能在抗凋亡中起作用。在这里，28个细胞和4个病毒蛋白质已被鉴定为进一步的可能的相互作用的tEBNA-LP的质谱。通过免疫沉淀和Western印迹证实了三种相互作用，即与PP 2A的A结构亚基（PP 2A A）、结构特异性识别蛋白1（SSRP 1，促进染色质转录（FACT）复合物的组分）和转录因子EC的新形式（TFEC）的相互作用。因此，tEBNA-LP似乎不仅通过与两个PP 2A亚基的相互作用参与细胞凋亡抗性，而且还参与其共激活转录调节因子的能力可能很重要的其他过程。
The Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) acts as a co-activator of EBNA-2, a transcriptional activator essential for Epstein-Barr virus (EBV)-induced B-cell transformation. Burkitt’s lymphoma (BL) cells harboring a mutant EBV strain that lacks both the EBNA-2 gene and 3′ exons of EBNA-LP express Y1Y2-truncated isoforms of EBNA-LP (tEBNA-LP) and better resist apoptosis than if infected with the wild-type virus. In such BL cells, tEBNA-LP interacts with the protein phosphatase 2A (PP2A) catalytic subunit (PP2A C), and this interaction likely plays a role in resistance to apoptosis. Here, 28 cellular and four viral proteins have been identified by mass spectrometry as further possible interactors of tEBNA-LP. Three interactions were confirmed by immunoprecipitation and Western blotting, namely with the A structural subunit of PP2A (PP2A A), the structure-specific recognition protein 1 (SSRP1, a component of the facilitate chromatin transcription (FACT) complex), and a new form of the transcription factor EC (TFEC). Thus, tEBNA-LP appears to be involved not only in cell resistance to apoptosis through its interaction with two PP2A subunits, but also in other processes where its ability to co-activate transcriptional regulators could be important.
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影响因子：
64.8
作者：
Bertolotto, Corine;Lesueur, Fabienne;Bressac-de Paillerets, Brigitte
通讯作者：
Bressac-de Paillerets, Brigitte
影响因子：
5.4
作者：
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影响因子：
82.9
作者：
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通讯作者：
Rickinson, A
DOI：
10.1007/978-1-62703-562-0_17
发表时间：
2013-01-01
期刊：
PHOSPHATASE MODULATORS
影响因子：
--
作者：
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通讯作者：
Janssens, Veerle
影响因子：
3.7
作者：
Kitay, MK;Rowe, DT
通讯作者：
Rowe, DT