Effects of disturbed flow on endothelial cells
Effects of disturbed flow on endothelial cells
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DOI：
10.1115/1.2834303
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发表时间：
1998-02-01
影响因子：
1.7
通讯作者：
Usami, S
中科院分区：
文献类型：
--
作者：
Chiu, JJ;Wang, DL;Usami, S
动脉粥样硬化病变倾向于定位在动脉系统的弯曲和分支处，其中局部流动经常被干扰和不规则（例如，流动分离、再循环、复杂的流动模式和不均匀的剪切应力分布）。采用垂直阶梯流动通道（VSF），研究了上述流动条件对体外培养的人脐静脉内皮细胞（HUVECs）的影响。在一般曲线坐标系下，用有限体积法计算了详细的切应力分布和流动结构。低切应力再附着区的HUVECs一般呈圆形。相比之下，在较高剪切应力下的细胞被显著拉长并且与流动方向对齐，甚至远离具有反向流动的区域中的那些。当HUVEC在VSF中受到剪切时，它们的肌动蛋白应力纤维会随着形态学变化而重组。流动再附着区附近的DNA合成速率高于层流区。这些体外实验为了解动脉粥样硬化病变流行区域中发现的复杂流动环境下内皮细胞的体内反应提供了数据。
Atherosclerotic lesions tend to localize at curvatures and branches of the arterial system, where the local flow is often disturbed and irregular (e.g., flow separation, recirculation, complex flow patterns, and nonuniform shear stress distributions). The effects of such flow conditions on cultured human umbilical vein endothelial cells (HUVECs) were studied in vitro by using a vertical-step flow channel (VSF). Detailed shear stress distributions and flow structures have been computed by using the finite volume method in a general curvilinear Coordinate system. HUVECs in the reattachment areas with low shear stresses were generally rounded in shape. fn contrast, the cells under higher shear stresses were significantly elongated and aligned with the flow direction, even far those in the area with reversed flow. When HUVECs were subjected to shearing in VSF, their actin stress fibers reorganized in association with the morphological changes. The rate of DNA synthesis in the vicinity of the flow reattachment area was higher than that in the laminar flow area. These in vitro experiments have provided data for the understanding of the in vivo responses of endothelial cells under complex flow environments found in regions of prevalence of atherosclerotic lesions.