Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device
Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device
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DOI：
10.1016/j.bios.2009.02.025
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发表时间：
2009-05-15
影响因子：
12.6
通讯作者：
Nishizawa, Matsuhiko
中科院分区：
文献类型：
--
作者：
Hashimoto, Masahiko;Kaji, Hirokazu;Nishizawa, Matsuhiko
我们描述了一种微流控方法，以选择性地捕获一种类型的白细胞（中性粒细胞或嗜酸性粒细胞）从悬浮液中的混合白细胞在一个特定的区域的微通道，其上壁配备了一个阵列的微带电极。其中一个微电极用于局部电生成次溴酸（HBrO），使通道的相对面具有蛋白吸附性。由于图案化层的聚（乙二醇）二甲基丙烯酸酯（PEGDM），不渗透HBrO，是预先引入在通道底部，蛋白质吸附的区域可以精确地定义，即使在存在的流体流动。将捕获抗体固定在处理区域上后，将AC电压（1 MHz，20 V-pp）施加到微电极阵列用于负介电泳（DEP），以从流动的细胞悬浮液中快速浓缩细胞。通过将嗜中性粒细胞或嗜酸性粒细胞特异性抗体固定到所创建的区域上，我们证明了可以从浓缩的白细胞中捕获相应的白细胞类型。(C)2009 Elsevier B. V.保留所有权利。
We describe a microfluidic method to selectively capture a type of leucocytes (neutrophils or eosinophils) from a suspension of mixed leucocytes in a specific region of a microchannel whose upper wall is equipped with an array of microband electrodes. One of the microelectrodes is used for locally electrogenerating hypobromous acid (HBrO) that renders the opposite face of the channel protein-adsorptive. Since the patterned layer of poly(ethylene glycol)dimethacrylate (PEGDM), impervious to HBrO, is previously introduced at the channel bottom, the region of protein adsorption can be precisely defined even in the presence of fluid-flow. After capture antibody was immobilized on the treated region, an AC voltage (1 MHz, 20 V-pp) was applied to the microelectrode array for negative dielectrophoresis (DEP) to quickly concentrate cells from the flowing cell suspension. By immobilizing neutrophil- or eosinophil-specific antibodies onto the created region, we demonstrated that the corresponding leucocyte type can be captured from the concentrated leucocytes. (C) 2009 Elsevier B.V. All rights reserved.