Spectrally encoded slit confocal microscopy.
Spectrally encoded slit confocal microscopy.
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DOI：
10.1364/ol.31.001687
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发表时间：
2006-06
期刊：
影响因子：
3.6
通讯作者：
Jeongmin Kim;Dongkyun Kang;D. Gweon
中科院分区：
文献类型：
--
作者：
Jeongmin Kim;Dongkyun Kang;D. Gweon
提出了一种简单且经济有效的共焦显微镜实时成像方法。光谱编码狭缝共焦显微镜 (SESCoM) 使用光谱编码技术和共焦狭缝孔径来获得二维图像。给出了 SESCoM 轴向和横向性能的仿真和实验结果。当使用NA为0.95的物镜时，测得的轴向响应的半高宽为1.15μm。沿 x 和 y 方向测得侧线扩展函数的半高宽分别为 236 和 244 nm。轴向和横向实验结果与模拟结果吻合良好。
A simple and cost-effective method for real-time imaging in confocal microscopy is proposed. Spectrally encoded slit confocal microscopy (SESCoM) uses a spectral encoding technique together with a confocal slit aperture to achieve two-dimensional images. Simulation and experimental results of the SESCoM's axial and lateral performances are presented. The measured FWHM of the axial response is 1.15 mum when an objective with a NA of 0.95 is used. FWHMs of the lateral line spread functions are measured to be 236 and 244 nm along the x and y directions, respectively. Both the axial and the lateral experimental results agree well with the simulation results.