Linkage mapping reveals loci that underlie differences in C. elegans growth
Linkage mapping reveals loci that underlie differences in C. elegans growth
复制标题
连锁图谱揭示了秀丽隐杆线虫生长差异背后的基因座
DOI：
10.1101/2022.04.25.489412
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Andersen, Erik C.
中科院分区：
文献类型：
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作者：
Nyaanga, Joy;Andersen, Erik C.
生长速度和身体大小是有助于生物体适应性的复杂特征。这些性状差异的基础基因座的鉴定提供了对发育的遗传贡献的见解。利用秀丽隐杆线虫作为数量遗传学的一个易处理的后生动物模型，我们可以确定生长差异的基因组区域。我们测量了实验室适应的野生型菌株（N2）和来自夏威夷的野生型菌株（CB 4856）的胚后生长，发现了体型的差异。利用连锁作图，我们确定了三个不同的数量性状位点（QTL）的染色体IV，V和X与身体生长的变化。我们进一步研究了这些生长相关的数量性状基因座使用染色体置换株系和近等基因系，并验证了X染色体数量性状基因座。此外，我们还生成了X染色体数量性状基因座的候选基因列表。这些基因可能导致动物生长的差异，应在后续研究中进行评估。我们的工作揭示了动物生长变异的遗传结构，并强调了秀丽隐杆线虫自然种群生长的遗传复杂性。
Growth rate and body size are complex traits that contribute to the fitness of organisms. The identification of loci that underlie differences in these traits provides insights into the genetic contributions to development. LeveragingCaenorhabditis elegansas a tractable metazoan model for quantitative genetics, we can identify genomic regions that underlie differences in growth. We measured postembryonic growth of the laboratory-adapted wild-type strain (N2) and a wild strain from Hawaii (CB4856) and found differences in body size. Using linkage mapping, we identified three distinct quantitative trait loci (QTL) on chromosomes IV, V, and X that are associated with variation in body growth. We further examined these growth-associated quantitative trait loci using chromosome substitution strains and near-isogenic lines and validated the chromosome X quantitative trait loci. In addition, we generated a list of candidate genes for the chromosome X quantitative trait loci. These genes could potentially contribute to differences in animal growth and should be evaluated in subsequent studies. Our work reveals the genetic architecture underlying animal growth variation and highlights the genetic complexity of growth inCaenorhabditis elegansnatural populations.