A micromethod for the estimation of blood dolichol.
A micromethod for the estimation of blood dolichol.
复制标题
一种测定血液多甘醇的微量方法。
作者：
Morris,GN;Pullarkat,RK
使用高效液相色谱法开发了一种测定血液中多醇的微量方法。该方法可应用于全血或血浆。将检测器灵敏度设置为 0.005 吸光度单位满量程时，只需 50 µl 的样品就足以进行测定。对 3、6、12 和 24 个月龄 C57 BL/6 NNia-1 小鼠近交系尾静脉的血液样本进行了检查。血液多甘醇水平随着年龄的增长而下降，从 6 个月时的 163.3 ng/ml 降至 24 个月时的 110.1 ng/ml。主要的多萜醇同系物是 C-85 (10.4%)、C-90 (41.6%)、C-95 (38.0%) 和 C-100 (8.1%)。
A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 μl are sufficient to carry out the determination. Blood samples from the tail vein of inbred strains of C57 BL/6 NNia‐1 mice 3,6, 12 and 24 mo of age were examined. Blood dolichol levels decreased with age from 163.3 ng/ml at 6 mo to 110.1 ng/ml at 24 mo. The major dolichol homologs were C‐85 (10.4%), C‐90 (41.6%), C‐95 (38.0%) and C‐100 (8.1%).