Novel genotyping system for distinguishing among native, non-native, and admixed individuals of rosy bitterling Rhodeus ocellatus subspecies
Novel genotyping system for distinguishing among native, non-native, and admixed individuals of rosy bitterling Rhodeus ocellatus subspecies
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用于区分玫瑰色苦味 Rhodeus ocellatus 亚种的本地、非本地和混合个体的新型基因分型系统
DOI：
10.1111/jfb.14333
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发表时间：
2020
影响因子：
2
通讯作者：
Onikura Norio
中科院分区：
文献类型：
--
作者：
Umemura Keitaro;Kurita Yoshihisa;Onikura Norio
2材料和方法我们接受福冈、佐贺县、熊本县的鱼类采集许可，日本没有动物福利法。所有的鱼都被轻轻地、快速地处理并立即释放到采样点。2.1研究设计我们的基因分型方法有三个步骤。首先，通过mtDNA分析确定母本DNA是来自r.o. kurumeus （mat-Rok）还是r.o. ocellatus （mat-Roo）。其次，nDNA分析确定材料是否属于以下三种类型之一：R. o. kurumeus-纯合（homo-Rok）， R. o. ocellatus-纯合（homo-Roo）或杂合（hetero）。最后，对这些结果进行综合分析，鉴定出了库鲁褐蝽、牛眼褐蝽和混合个体。理论上，该方法可检测出6种基因型：库鲁默氏r.o.个体为mat-Rok和homo-Rok；红腹小腹的个体为席- roo型和同- roo型；和混合个体是mat-Rok与hetero或homo-Roo，或mat-Roo与hetero或homo-Rok（支持信息图S1）。
2 MATERIALS AND METHODSWe accept permission for fish collections by Fukuoka, Saga, Kumamoto prefectures and there are no animal welfare laws in Japan. All fishes were gently and quickly treated and immediately released at the sampling sites.2.1 Study designOur genotyping method has three steps. First, an mtDNA analysis determines whether the maternal DNA is from R. o. kurumeus (mat-Rok) or R. o. ocellatus (mat-Roo). Second, an nDNA analysis identifies if the material is of one of these three types: R. o. kurumeus-homozygosity (homo-Rok), R. o. ocellatus-homozygosity (homo-Roo) or heterozygosity (hetero). Finally, these results are combined and R. o. kurumeus, R. o. ocellatus and admixed individuals are identified. In theory, this method can detect six genotypes: R. o. kurumeus individuals are mat-Rok and homo-Rok; R. o. ocellatus individuals are mat-Roo and homo-Roo; and admixed individuals are mat-Rok with hetero or homo-Roo, or mat-Roo with hetero or homo-Rok (Supporting Information Figure S1).