Paclitaxel-conjugated PEG and arginine-grafted bioreducible poly (disulfide amine) micelles for co-delivery of drug and gene.
Paclitaxel-conjugated PEG and arginine-grafted bioreducible poly (disulfide amine) micelles for co-delivery of drug and gene.
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DOI：
10.1016/j.biomaterials.2012.07.031
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发表时间：
2012-11
期刊：
影响因子：
14
通讯作者：
Kim, Sung Wan
中科院分区：
文献类型：
--
作者：
Kihoon Nam;Hye Yeong Nam;Kim, Pyung-Hwan;Kim, Sung Wan
我们研制了一种紫杉醇偶联聚合物胶束，ABP-PEG3.5K-紫杉醇(APP)，它由聚乙二醇(PEG)和精氨酸接枝的聚半胱胺双丙烯酰胺-二氨基己烷(ABP)组成，用于基因和药物的共传递。APP聚合物自组装成阳离子聚合物胶束，其临界胶束浓度(Cmc)约为0.062 mg/m L。胶束的平均尺寸约为3 nm，Zeta电位约为+14 mV。由于表面带正电荷，APP胶束与直径约200 nm的质粒DNA形成多聚体。荧光素酶基因和小鼠白介素12(IL-12)基因用于监测基因传递能力。与单独使用紫杉醇相比，APP复合体显示出更高的基因传递效率和细胞摄取，并具有更高的抗癌效力。这些结果表明，基于APP胶束的递送系统非常适合于基因和药物的共递送。
We developed a paclitaxel-conjugated polymeric micelle, ABP-PEG3.5k-Paclitaxel (APP) consisting of poly (ethylene glycol) (PEG) and arginine-grafted poly(cystaminebisacrylamide-diaminohexane) (ABP) for the co-delivery of gene and drug. The APP polymer self-assembled into cationic polymeric micelles with a critical micelle concentration (CMC) value of approximately 0.062 mg/mL, which was determined from measurements of the UV absorption of pyrene. The micelles have an average size of about 3 nm and a zeta potential of about +14 mV. Due to the positive surface charge, APP micelles formed polyplexes with plasmid DNA approximately 200 nm in diameter. The luciferase gene and mouse interleukin-12 (IL-12) gene was used to monitor gene delivery potency. APP polyplexes showed increased gene delivery efficiency and cellular uptake with higher anticancer potency than paclitaxel alone. These results demonstrate that an APP micelle-based delivery system is well suitable for the co-delivery of gene and drug.
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