pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface.
pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface.
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DOI：
10.1016/j.colsurfb.2004.11.002
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
Ning Fang;W. Tan;K. Leong;H. Mao;V. Chan
中科院分区：
文献类型：
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作者：
Ning Fang;W. Tan;K. Leong;H. Mao;V. Chan
聚合物支撑脂质双层膜是设计和制备新型仿生器件的关键技术。迄今为止，形成聚合物支撑的脂质双层的物理驱动力仍有待确定。在这项研究中，二棕榈酰磷酸胆碱（DPPC）囊泡和聚（对苯二甲酸乙二醇酯）[PET]表面有或没有接枝聚（丙烯酸）[PAA]层之间的相互作用与几种生物物理技术检查。首先，囊泡变形分析表明，无论是平原PET或PAA接枝PET表面上的粘附囊泡的几何形状是最好的描述截断球模型。在中性pH值下，丙烯酸在PET表面的接枝聚合不改变PET的变形程度和粘附能。有趣的是，在室温下，随着pH值的增加，PAA接枝PET和普通PET表面的粘附能平均值分别增加了185%和−43%。我们的研究结果表明，通过控制固体载体上的电离，可以调节囊泡和聚合物垫之间的粘附相互作用。
Polymer-supported lipid bilayer is a key enabling technology for the design and fabrication of novel biomimetic devices. To date, the physical driving force underlying the formation of polymer-supported lipid bilayer remains to be determined. In this study, the interaction between dipalmitoylphosphocholine (DPPC) vesicle and poly(ethylene terephthalate) [PET] surface with or without grafted poly(acrylic acid) [PAA] layer is examined with several biophysical techniques. First, vesicle deformation analysis shows that the geometry of adherent vesicle on either plain PET or PAA-grafted PET surface is best described by a truncated sphere model. At neutral pH, the degree of deformation and adhesion energy are unaltered by the grafted polymerization of acrylic acid on PET surface. Interestingly, the average magnitude of adhesion energy is increased by 185% and −43% on PAA-grated PET and plain PET surface, respectively, towards an increase of pH at room temperature. Our results demonstrate the possibility of tuning the adhesive interaction between vesicle and polymer cushion through the control of polyelectrolyte ionization on the solid support.