Microbial activity and metamitron degrading microbial communities differ between soil and water-sediment systems.
Microbial activity and metamitron degrading microbial communities differ between soil and water-sediment systems.
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DOI：
10.1016/j.jhazmat.2020.124293
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发表时间：
2020-10
影响因子：
13.6
通讯作者：
S. Wang;A. Miltner;A. M. Muskus;K. Nowak
中科院分区：
文献类型：
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作者：
S. Wang;A. Miltner;A. M. Muskus;K. Nowak
除草剂苯草胺在环境中经常被检测到，其在土壤中的降解不同于在水生沉积物中的降解。本研究应用13 C6-苯并咪唑研究了土壤和水-沉积物系统中微生物活性、苯并咪唑矿化和苯并咪唑降解微生物群落的差异。与对照组相比，Metamitron增加了土壤呼吸，而它抑制了水-沉积物系统中的呼吸。Metamitron在土壤中的矿化速度比在水沉积物中快两倍。土壤中13 C6-苯并咪唑的13 C掺入磷脂脂肪酸（PLFAs）的比例高于沉积物，说明土壤中苯并咪唑降解微生物的活性较高。在偏苯脒加速矿化过程中，土壤中13 C-PLFA主要是革兰氏阴性菌、革兰氏阳性菌和放线菌的生物标志物。革兰氏阴性菌占主导地位的代谢产物在沉积物中的整个潜伏期。放线菌、放线菌和真菌分别是土壤和水-沉积物中初级降解物的主要消耗者。这项研究清楚地表明，参与metamitron降解的微生物群体取决于系统（土壤与水沉积物）和时间。它还表明，有机化学品在复杂环境中的周转是由不同的合成降解物（初级降解物和坏死物质降解物），而不是由一个单一的降解物。
The herbicide metamitron is frequently detected in the environment, and its degradation in soil differs from that in aquatic sediments. In this study, we applied13C6-metamitron to investigate the differences in microbial activity, metamitron mineralization and metamitron degrading microbial communities between soil and water-sediment systems. Metamitron increased soil respiration, whereas it suppressed respiration in the water-sediment system as compared to controls. Metamitron was mineralized two-fold faster in soil than in the water-sediment. Incorporation of13C from13C6-metamitron into Phospholipid fatty acids (PLFAs) was higher in soil than in sediment, suggesting higher activity of metamitron-degrading microorganisms in soil. During the accelerated mineralization of metamitron, biomarkers for Gram-negative, Gram-positive bacteria and actinobacteria dominated within the13C-PLFAs in soil. Gram-negative bacteria dominated among the metamitron degraders in sediment throughout the incubation period. Actinobacteria, and actinobacteria and fungi were the main consumers of necromass of primary degraders in soil and water-sediment, respectively. This study clearly showed that microbial groups involved in metamitron degradation depend on the system (soil vs. water-sediment) and on time. It also indicated that the turnover of organic chemicals in complex environments is driven by different groups of synthropic degraders (primary degraders and necromass degraders) rather than by a single degrader.