Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels
Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels
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DOI：
10.1002/mabi.200900410
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发表时间：
2010-08-11
影响因子：
4.6
通讯作者：
Milani, Paolo
中科院分区：
文献类型：
--
作者：
Dos Reis, Gabriel;Fenili, Fabio;Milani, Paolo
细胞模式是组织细胞表面和以简单的方式再现多细胞生命的组织层次和复杂性的重要工具。因此，控制固体表面上的细胞生长、增殖和分化对于假肢、生物传感器、细胞阵列、干细胞治疗和基于细胞的药物发现概念非常重要。我们提出了一种新的电子束光刻方法，用于在生物相容性和可生物降解的PAA水凝胶上直接和同时制造亚微米的地形和化学图案。局部电子束修饰的水凝胶表面使图案能够吸附蛋白质，与防污表面形成对比。通过利用PC12细胞的选择性附着、生长和分化，我们构建了一个由沿微通道延伸的神经炎连接的单细胞神经网络。电子束微光刻技术在PAA水凝胶上开辟了生产包含复杂地形的多功能微器件的机会，允许精确控制单个细胞的生长和组织。
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.