A versatile, low cost light source module for multiphoton imaging
A versatile, low cost light source module for multiphoton imaging
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用于多光子成像的多功能、低成本光源模块
DOI：
10.1117/12.2601570
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bourdakos K
中科院分区：
文献类型：
--
作者：
Bourdakos K
多光子成像方法，如相干拉曼散射（CRS）显微镜，也包括二次谐波产生（SHG）和双光子激发自动荧光（TPEAF）成像（称为多模态相干拉曼显微镜），由于其独特的特点，极大地促进了生物医学研究的进步。多模态CRS显微镜，无标签，化学特异性，固有的“共聚焦”，提供三种独立的对比机制，可以在包含有关被检测样品的广泛化学和结构信息的合成图像中相关联。多模态CRS显微镜的标准光源是皮秒泵浦光学参量振荡器（OPO），其表现出优异的性能，但由于其相关的高成本、维护、复杂性和对专用光学实验室的要求，阻碍了多模态CRS显微镜的广泛采用，特别是在临床应用中的部署。本文提出了一种新型的、低成本的光学参量放大器（OPA），该放大器基于MgO掺杂的周期性极化铌酸锂（PPLN）晶体，该晶体由连续波（CW）激光源播种，并由皮秒激光在1031nm处泵浦，从而消除了任何同步要求。我们表明，这种OPA是一种多功能光源模块，可以根据特定应用的可调性和可负担性要求进行定制。我们证明了它既可以与OPO结合使用，也可以单独用作多模态CRS显微镜的光源，并通过对各种标准和生物样品进行成像来展示其性能。
Multiphoton imaging methods such as Coherent Raman Scattering (CRS) microscopy which also comprises Second Harmonic Generation (SHG) and Two Photon Excited Auto-Fluorescence (TPEAF) imaging (termed as multimodal Coherent Raman microscopy), have greatly facilitated the advancement of biomedical research due to their unique features. Multimodal CRS microscopy, is label free, chemically specific, inherently ‘confocal’ offering three independent contrast mechanisms which can be associated in a composite image comprising a wide range of chemical and structural information about the interrogated sample. The standard light source for multimodal CRS microscopy is a picosecond pumped Optical Parametric Oscillator (OPO) which has exhibited excellent performance but due to its associated high cost, maintenance, complexity and requirement of a dedicated optics laboratory, has hindered the wider adoption of multimodal CRS microscopy and especially its deployment in clinical applications. Here we present a novel, low cost Optical Parametric Amplifier (OPA) based on a MgO doped Periodically Poled Lithium Niobate (PPLN) crystal seeded by a continuous wave (CW) laser source and pumped by a picosecond laser at 1031nm, which removes any synchronisation requirements. We show that this OPA is a versatile light source module that can be tailored to the tunability and affordability requirements of the specific application. We demonstrate that it can be used either in association with an OPO or on its own as a light source for multimodal CRS microscopy and we show its performance by imaging a variety of standards and biological samples.
超快光学中均方根宽度和均方根啁啾的测量
DOI：
--
发表时间：
2000
期刊：
影响因子：
--
作者：
E. Sorokin;G. Tempea;T. Brabec
通讯作者：
T. Brabec