Characterization of the DA26 gene in a hypervariable region of the Autographa californica nuclear polyhedrosis virus genome.
Characterization of the DA26 gene in a hypervariable region of the Autographa californica nuclear polyhedrosis virus genome.
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苜蓿银纹夜蛾核型多角体病毒基因组高变区 DA26 基因的表征。
DOI：
10.1099/0022-1317-71-5-1029
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Miller,LK
中科院分区：
文献类型：
--
作者：
O'Reilly,DR;Passarelli,AL;Goldman,IF;Miller,LK
杆状病毒苜蓿银纹夜蛾核多角体病毒基因组的一个区域在细胞培养物中连续传代后经常被发现发生改变。对野生型和突变病毒基因组该区域的序列分析表明，一些突变影响了 675 bp 的开放阅读框，称为 DA26。 DA26 基因通过删除和插入类似转座元件的序列而被破坏。 DA26 的 Northern 印迹分析表明它在感染后很早就表达。感染培养的粉纹夜蛾后，经过 1 小时的病毒吸附期后，可以检测到 DA26 特异性转录本。这些转录本通过核酸酶保护测定进行绘制。构建了重组病毒，其中通过插入大肠杆菌lacZ基因来破坏DA26。这种病毒在两种T细胞中均能存活。对草地贪夜蛾细胞和蛋白质合成动力学的分析表明野生型病毒和重组病毒之间没有差异。 DA26 的破坏也不会干扰病毒感染 T 的能力。尼奥尔S。果蝇幼虫。
A region of the baculovirusAutographa californicanuclear polyhedrosis virus genome that is frequently found to be altered after serial passage of the virus in cell culture was characterized. Sequence analysis of this region of the genome in wild-type and mutant viruses revealed that some of the mutations affected a 675 bp open reading frame, designated DA26. The DA26 gene was disrupted both by deletion and by insertion of sequences that resembled transposable elements. Northern blot analysis of DA26 showed that it was expressed very early after infection. DA26-specific transcripts could be detected after the 1 h viral adsorption period upon infection of culturedTricho- plusia nicells. These transcripts were mapped by nuclease protection assays. A recombinant virus was constructed in which DA26 was disrupted by insertion of theEscherichia coli lacZgene. This virus was viable in bothT. niandSpodoptera frugiperdacells and analysis of the kinetics of protein synthesis revealed no differences between wild-type and recombinant viruses. The disruption of DA26 also did not interfere with the ability of the virus to infectT. niorS. frugiperdalarvae.