Detection and Genotype of High-Risk Human Papillomavirus in Fine-Needle Aspirates of Patients With Metastatic Squamous Cell Carcinoma Is Helpful in Determining Tumor Origin
Detection and Genotype of High-Risk Human Papillomavirus in Fine-Needle Aspirates of Patients With Metastatic Squamous Cell Carcinoma Is Helpful in Determining Tumor Origin
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DOI：
10.1309/ajcpcza4pszcfhq4
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发表时间：
2015-05-01
影响因子：
3.5
通讯作者：
Chhieng, David
中科院分区：
文献类型：
--
作者：
Baldassarri, Rebecca;Aronberg, Ryan;Chhieng, David
目的：最近的研究表明，人乳头状瘤病毒（HPV）与头颈部鳞状细胞癌（HNSCC）的某些子集有关-即那些发生在口咽部的鳞状细胞癌。本研究的目的是使用罗氏cobas 4800系统确定高危型HPV的有效性、检测和基因型（罗氏分子系统，加利福尼亚州普莱森顿）。方法：来自37例颈部（n = 36）或纵隔（n = 5）淋巴结病或左咽旁肿块（n = 1）患者的42份细针穿刺（FNA）标本被纳入这项前瞻性研究。直接涂片制备后，对残留的FNA材料进行HR-HPV检测，如果阳性，则使用Roche cobas 4800系统进一步描述HPV 16/18基因型。结果：在HR-HPV阳性的HNSCC中，18例（100%）起源于口咽部，15例HR-HPV阴性的HNSCC中仅有2例（13%）起源于口咽部（χ 2检验，P <0.05）。p16免疫组化和HPV 16原位杂交检测结果与细胞学HR-HPV检测结果一致。结论：采用Roche cobas 4800系统可对转移性鳞癌淋巴结FNAs进行HR-HPV检测和基因分型。HR-HPV和/或HPV 16的存在是来源于口咽部的转移性鳞状细胞癌的可靠指标。
Objectives: Recent studies have shown that human papillomavirus (HPV) is associated with a certain subset of head and neck squamous cell carcinoma (HNSCC)-namely, those arising in the oropharynx. The objective of this study is to determine the efficacy, detection, and genotype of high-risk (HR) HPV using the Roche cobas 4800 system (Roche Molecular System, Pleasanton, CA).Methods: Forty-two fine-needle aspirate (FNA) specimens from 37 patients with cervical (n = 36) or mediastinal (n = 5) lymphadenopathy or a left parapharyngeal mass (n = 1) were included in this prospective study. HR-HPV testing was performed on residual FNA material after direct smear preparation and, if positive, was further delineated into HPV 16/18 genotypes using the Roche cobas 4800 system. Follow-up included review of histologic material and/or electronic health records.Results: Among those HNSCCs that were positive for HR-HPV, 18 (100%) of 18 originated from the oropharynx, whereas only two (13%) of 15 HR-HPV negative HNSCCs originated from the oropharynx (chi(2) test, P < .05). p16 immunohistochemical assay and HPV 16 in situ hybridization on corresponding histologic specimens were concordant with cytologic HR-HPV results.Conclusions: HR-HPV detection and genotyping can be performed on lymph node FNAs with metastatic squamous cell carcinoma using the Roche cobas 4800 system. The presence of HR-HPV and/or HPV 16 is a reliable indicator of the metastatic squamous cell carcinoma originating from the oropharynx.