The identification and location of succinyl residues and the characterization of the interior arabinan region allow for a model of the complete primary structure of Mycobacterium tuberculosis mycolyl arabinogalactan
The identification and location of succinyl residues and the characterization of the interior arabinan region allow for a model of the complete primary structure of Mycobacterium tuberculosis mycolyl arabinogalactan
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DOI：
10.1074/jbc.m800222200
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发表时间：
2008-05-09
影响因子：
4.8
通讯作者：
McNeil, Michael R.
中科院分区：
文献类型：
--
作者：
Bhamidi, Suresh;Scherman, Michael S.;McNeil, Michael R.
结核分枝杆菌的复杂细胞壁是抗酸细菌的标志，并负责其许多生理特性。因此，已经做了很多努力来确定其初级结构。这种研究因其极端复杂性而受到阻碍。此外，它的不溶性导致难以确定是否存在碱基不稳定基团。我们已经使用内源性阿拉伯糖酶来溶解细胞壁的阿拉伯聚糖区域，并且已经使用质谱法和NMR显示琥珀酰酯存在于内部分支的1，3，5-α-D-阿拉伯呋喃糖基残基的O2上。此外，已鉴定出14个线性α-1，5阿拉伯呋喃糖基残基的内部阿拉伯聚糖区域。这些和早期的结果，现在允许提出一个模型的分枝杆菌分枝菌酰阿拉伯半乳聚糖的整个一级结构突出了三个阿拉伯聚糖链的31个残基。
The complex cell wall of Mycobacterium tuberculosis is the hallmark of acid fast bacteria and is responsible for much of its physiological characteristics. Hence, much effort has been made to determine its primary structure. Such studies have been hampered by its extreme complexity. Also, its insolubility leads to difficulties determining the presence or absence of base labile groups. We have used an endogenous arabinase to solubilize the arabinan region of the cell wall and have shown using mass spectrometry and NMR that succinyl esters are present on O2 of the inner-branched 1,3,5-alpha-D-arabinofuranosyl residues. In addition, an inner arabinan region of 14 linear alpha-1,5 arabinofuranosyl residues has been identified. These and earlier results now allow the presentation of a model of the entire primary structure of the mycobacterial mycolyl arabinogalactan highlighted by three arabinan chains of 31 residues each.