Determination of tyrosine, tryptophan and their metabolic derivatives by liquid chromatography-electrochemical detection: application to post mortem samples from patients with Parkinson's and Alzheimer's disease.
Determination of tyrosine, tryptophan and their metabolic derivatives by liquid chromatography-electrochemical detection: application to post mortem samples from patients with Parkinson's and Alzheimer's disease.
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液相色谱-电化学检测法测定酪氨酸、色氨酸及其代谢衍生物：应用于帕金森病和阿尔茨海默病患者的尸检样本。
DOI：
10.1016/0024-3205(85)90257-7
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发表时间：
1985
期刊：
影响因子：
6.1
通讯作者：
Slevin,JT
中科院分区：
文献类型：
--
作者：
Sparks,DL;Slevin,JT
本文介绍了一种快速测定主要吲哚类和邻苯二酚类化合物的方法。皮克检测限的分析是通过使用电化学检测器（LCEC）的C18反相柱上的高压液相色谱法进行的。该方法提供了一个全面的列表，可以同时测定脑样品中的化合物，其中没有衍生化或柱前纯化的必要。9个神经化学物质的区域分布从大鼠脑和10个神经化学物质的水平从人脑中提出。在尾状核和壳核中检测到DOPA、TYR、NE、MHPG、DOPAC、5-HIAA、TRP、DA、HVA、3-MT和5-HT。帕金森氏病痴呆患者的尾状核和壳核的神经化学物质的水平急剧下降，儿茶酚和吲哚损失最大的壳核。阿尔茨海默病（SDAT）患者的尾状核和壳核中的神经化学物质水平也急剧下降; NE的损失仅见于壳核，DA，HVA和5-HT的损失在尾状核和壳核中是均匀的。SDAT患者脑脊液中仅NE改变。
A procedure is described for the rapid determination of the major indoles and catechols. Analysis with picogram detection limits was done by high-pressure liquid chromatography on a C18 reverse-phase column using electrochemical detection (LCEC). This method provides a comprehensive list of compounds which can be simultaneously determined in brain samples and for which there is no necessity of derivatization or pre-column purification.The regional distribution of 9 neurochemicals from rat brain and the levels of 10 neurochemicals from human brain are presented. DOPA, TYR, NE, MHPG, DOPAC, 5-HIAA, TRP, DA, HVA, 3-MT and 5-HT were detected in the caudate nucleus and putamen. The levels of neurochemicals from the caudate and putamen of a demented patient with Parkinson's disease were variably decreased; catechol and indole losses were greatest in the putamen. The levels of neurochemicals in the caudate and putamen of patients with Alzheimer's disease (SDAT) were also variably decreased; loss of NE was seen only in putamen and losses of DA, HVA and 5-HT were uniform across both caudate and putamen. The CSF of SDAT patients showed changes in NE only.