Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae.
Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae.
复制标题
马铃薯洋葱连作增强番茄对大丽黄萎病的抗病能力
DOI：
10.3389/fpls.2015.00726
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发表时间：
2015
影响因子：
5.6
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Fu X;Wu X;Zhou X;Liu S;Shen Y;Wu F
间作可以缓解土传病害，但对种间相互作用诱导寄主植物免疫的研究较少。为了验证间作是否能提高寄主植物的抗病能力，研究了马铃薯洋葱与马铃薯洋葱伴作对番茄黄萎病的防治效果。为探讨其作用机制，分别采集番茄和马铃薯洋葱的根系分泌物，对其在体外对大丽花弧菌的抑菌活性进行了研究。此外，利用RNA-seq技术检测了在大丽花弧菌侵染条件下，与单独生长的番茄相比，与马铃薯洋葱伴生的番茄抗病相关基因的表达谱。结果表明，马铃薯洋葱配作能减轻番茄黄萎病的发生和严重程度。进一步研究发现，番茄和马铃薯洋葱根系分泌物对大丽花病原菌菌丝生长和孢子萌发有显著抑制作用。然而，单独种植的马铃薯洋葱或与番茄一起种植的马铃薯洋葱根系分泌物对这两项测量没有显著影响。RNA-seq数据分析显示，与马铃薯洋葱伴生的番茄中，与致病相关蛋白、木质素生物合成、激素代谢和信号转导相关的疾病防御基因表达量远高于单独生长的番茄，表明这些防御基因在番茄抗大丽花弧菌感染中发挥了重要作用。说明番茄与马铃薯洋葱配合处理，对大丽花病菌的抗性增强。我们认为马铃薯洋葱配种可以通过调控抗病应答相关基因的表达来增强番茄对大丽花病菌的抗性。这可能是间作系统土壤传播植物病害管理的潜在机制。
Intercropping could alleviate soil-borne diseases, however, few studies focused on the immunity of the host plant induced by the interspecific interactions. To test whether or not intercropping could enhance the disease resistance of host plant, we investigated the effect of companion cropping with potato onion on tomato Verticillium wilt caused by Verticillium dahliae (V. dahliae). To investigate the mechanisms, the root exudates were collected from tomato and potato onion which were grown together or separately, and were used to examine the antifungal activities against V. dahliae in vitro, respectively. Furthermore, RNA-seq was used to examine the expression pattern of genes related to disease resistance in tomato companied with potato onion compared to that in tomato grown alone, under the condition of infection with V. dahliae. The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt. The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae. However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato. RNA-seq data analysis showed the disease defense genes associated with pathogenesis-related proteins, biosynthesis of lignin, hormone metabolism and signal transduction were expressed much higher in the tomato companied with potato onion than those in the tomato grown alone, which indicated that these defense genes play important roles in tomato against V. dahliae infection, and meant that the disease resistance of tomato against V. dahliae was enhanced in the companion copping with potato onion. We proposed that companion cropping with potato onion could enhance the disease resistance of tomato against V. dahliae by regulating the expression of genes related to disease resistance response. This may be a potential mechanism for the management of soil-borne plant diseases in the intercropping system.