SARS-CoV-2 in river water: Implications in low sanitation countries
SARS-CoV-2 in river water: Implications in low sanitation countries
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DOI：
10.1016/j.scitotenv.2020.140832
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发表时间：
2020-11-15
影响因子：
9.8
通讯作者：
Rios-Touma, Blanca
中科院分区：
文献类型：
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作者：
Guerrero-Latorre, Laura;Ballesteros, Isabel;Rios-Touma, Blanca
自2019冠状病毒病大流行开始以来，关于病毒脱落的研究报告称，这种病毒在大多数患者的粪便中排出。在COVID-19病例数量较多的城市，在污水管道或污水处理厂入口处发现高病毒载量。在基多（厄瓜多尔）和世界上许多其他城市一样，废水直接排入自然沃茨。本研究的目的是评估SARS-CoV-2在低卫生条件下的城市溪流中的存在。6月5日，在COVID-19病例高峰期，对基多城市河流沿线沿着的三条河流进行了采样。河水样本的水质参数进行了评估，然后，浓缩的病毒分析使用脱脂牛奶絮凝法。对病毒浓缩物中的SARS-CoV-2（N1和N2靶区）和人腺病毒（作为人病毒指示剂）进行定量。结果显示，在N1的2，91 E +05至3，19 E +06 GC/L和N2的2，07 E +05至2，22 E +06 GC/L范围内，在所有分析样本的两个靶区域均检测到SARS-CoV-2。在低卫生区域的天然沃茨中检测到的高值对健康和生态有若干影响，应进一步评估。(C)2020年，任作家。由爱思唯尔公司出版
Since the beginning of COVID-19 pandemic studies on viral shedding have reported that this virus is excreted in feces in most patients. High viral loads are found at the sewage pipeline or at the entrance of wastewater treatment plants from cities where the number of COVID-19 cases are significant. In Quito (Ecuador) as in many other cities worldwide, wastewater is directly discharged into natural waters. The aim of this study was to evaluate SARS-CoV-2 presence in urban streams from a low sanitation context. Three river locations along the urban rivers of Quito were sampled on the 5th of June during a peak of COVID-19 cases. River samples were evaluated for water quality parameters and afterwards, concentrated for viral analysis using skimmed milk flocculation method. The viral concentrates were quantified for SARS-CoV-2 (N1 and N2 target regions) and Human Adenovirus as a human viral indicator. The results showed that SARS-CoV-2 was detected for both target regions in all samples analyzed in a range of 2,91E+05 to 3,19E+06 GC/L for N1 and from 2,07E+05 to 2,22E+06 GC/L for N2. The high values detected in natural waters from a low sanitation region have several implications in health and ecology that should be further assessed. (C) 2020 The Authors. Published by Elsevier B.V.