Cyclodextrin-Based Metal-Organic Nanotube as Fluorescent Probe for Selective Turn-On Detection of Hydrogen Sulfide in Living Cells Based on H2S-Involved Coordination Mechanism.
Cyclodextrin-Based Metal-Organic Nanotube as Fluorescent Probe for Selective Turn-On Detection of Hydrogen Sulfide in Living Cells Based on H2S-Involved Coordination Mechanism.
复制标题
基于环糊精的金属有机纳米管作为荧光探针，基于 H2S 参与的配位机制选择性开启检测活细胞中的硫化氢
DOI：
10.1038/srep21951
复制
发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Xin X;Wang J;Gong C;Xu H;Wang R;Ji S;Dong H;Meng Q;Zhang L;Dai F;Sun D
硫化氢被认为是继一氧化氮(NO)和一氧化碳(CO)之后的第三种生物气体信使(气体递质)。对活细胞中硫化氢的荧光检测对人类健康非常重要，因为已经发现人体内硫化氢水平的异常会导致阿尔茨海默病、癌症和糖尿病。在这里，我们开发了一种基于环糊精的金属-有机纳米管CD-Mont-2，它具有{Pb14}金属大环，可以有效地检测硫化氢。Cd-Mont-2‘(无客体形式的Cd-Mont-2)在DMSO溶液中对H_2S具有高选择性和中等灵敏度的开启检测。值得注意的是，CD-Mont-2‘可以作为荧光开启的探针，高度选择性地检测活细胞中的硫化氢。本工作的传感机理是基于H_2S作为辅色基团与中心的铅(II)离子配位来增强荧光强度，这是首次研究的。
Hydrogen sulfide (H2S) has been considered as the third biologically gaseous messenger (gasotransmitter) after nitric oxide (NO) and carbon monoxide (CO). Fluorescent detection of H2S in living cells is very important to human health because it has been found that the abnormal levels of H2S in human body can cause Alzheimer’s disease, cancers and diabetes. Herein, we develop a cyclodextrin-based metal-organic nanotube, CD-MONT-2, possessing a {Pb14} metallamacrocycle for efficient detection of H2S. CD-MONT-2′ (the guest-free form of CD-MONT-2) exhibits turn-on detection of H2S with high selectivity and moderate sensitivity when the material was dissolved in DMSO solution. Significantly, CD-MONT-2′ can act as a fluorescent turn-on probe for highly selective detection of H2S in living cells. The sensing mechanism in the present work is based on the coordination of H2S as the auxochromic group to the central Pb(II) ion to enhance the fluorescence intensity, which is studied for the first time.