Regulation of human natural killing by lysosomotropic and thiol-reactive agents.
Regulation of human natural killing by lysosomotropic and thiol-reactive agents.
复制标题
溶酶体和硫醇反应剂对人类自然杀伤的调节。
作者：
H. Shau;J. Dawson
用铵或其它亲溶酶体胺处理人外周血淋巴细胞（PBL）消除效应细胞的自然杀伤（NK）活性而不影响其活力。这些试剂对NK的抑制可能是由于在效应细胞靶细胞结合之后但在细胞溶解编程之前阻断了事件。用相同的试剂处理K562靶细胞对靶细胞对NK的敏感性没有影响。用NK抑制性亲溶酶体剂预处理效应细胞可降低溶酶体硫醇组织蛋白酶活性，而不影响溶酶体酸性磷酸酶活性。巯基反应剂，甲苯磺酰基-L-赖氨酸氯甲基酮（TLCK）和碘乙酰胺也抑制PBL中的NK和巯基组织蛋白酶活性。这些数据表明，含巯基的酶在NK细胞溶解的早期阶段的作用。
Treatment of human peripheral blood lymphocytes (PBL) with ammonium or other lysosomotropic amines eliminates natural killing (NK) activity of effector cells without affecting their viability. Inhibition of NK by these reagents is probably due to blocking of events subsequent to effector cell target cell binding but before programming for cytolysis. Treatment of K562 target cells with the same reagents has no effect on target cell sensitivity to NK. Pretreatment of effector cells with the NK inhibitory lysosomotropic agents decreases lysosomal thiol cathepsin activity without affecting the lysosomal acid phosphatase activity. Thiol-reactive agents, tosyl-L-lysine chloromethyl ketone (TLCK) and iodoacetamide also inhibit NK and thiol cathepsin activity in PBL. These data suggest a role for thiol-containing enzymes in the early stages of NK cytolysis.