Digital microfluidics for cell-based assays
Digital microfluidics for cell-based assays
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DOI：
10.1039/b717759c
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Wheeler, Aaron R.
中科院分区：
文献类型：
--
作者：
Barbulovic-Nad, Irena;Yang, Hao;Wheeler, Aaron R.
我们引入了一种实施基于细胞的测定的新方法。该方法基于数字微流体 (DMF)，用于驱动电极平面阵列上的纳升试剂和细胞液滴。我们证明，这种方法对于基于细胞的测定是有利的，因为除了减少试剂使用和分析时间之外，还可以自动操作多种试剂。在细胞活力、增殖和生物化学测定中未观察到 DMF 驱动的不利影响。新方法使用 Jurkat T 细胞进行细胞毒性测定，其灵敏度比传统孔板测定高出 20 倍。这些结果表明 DMF 作为一种简单而通用的分析工具具有巨大的潜力，可用于在微尺度上实施基于细胞的测定。
We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. A cytotoxicity assay using Jurkat T-cells was performed using the new method, which had similar to 20 times higher sensitivity than a conventional well plate assay. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays on the microscale.