Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: Ultrastructural demonstration of herpesvirus in lymphoma cells
Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: Ultrastructural demonstration of herpesvirus in lymphoma cells
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DOI：
10.1182/blood.v87.12.4937.bloodjournal87124937
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发表时间：
1996-06-15
期刊：
影响因子：
20.3
通讯作者：
Koeffler, HP
中科院分区：
文献类型：
--
作者：
Said, JW;Chien, K;Koeffler, HP
最近的分子证据表明，一种新的疱疹病毒，卡波西肉瘤相关疱疹病毒(KSHV/HHV-8)和原发性渗出性淋巴瘤(PEL)有关。PEL具有特征性的形态、表型和临床表现，在没有毗连的实体瘤肿块的情况下伴有恶性积液。我们从KSHV阳性的人类免疫缺陷病毒(HIV)阴性的胸膜腔淋巴瘤患者身上建立了一种细胞系(KS-1)，并将其传给了三重免疫缺陷的BNX小鼠。与同时含有KSHV和Epstein Barr病毒基因组的来自HIV阳性患者的体腔淋巴瘤细胞系相比，这些细胞只含有KSHV，从而允许进行未受污染的病毒学研究。超微结构检查发现恶性细胞具有晚期分化B细胞(免疫母细胞)的特征。具有病毒细胞病变效应的细胞含有典型的110 nm核内疱疹病毒核衣壳和完整的细胞质病毒粒子，证实了PEL与KSHV的关联。(C)1996年由美国血液病学会主办。
Recent molecular evidence suggests an association with a new herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8), and primary effusion lymphomas (PELs). PELs have a characteristic morphology, phenotype, and clinical presentation, with malignant effusions in the absence of a contiguous solid tumor mass. We have established a cell line (KS-1) from a KSHV-positive human immunodeficiency virus (HIV)-negative patient with pleural cavity-based lymphoma that was passaged into triple-immunodeficient BNX mice. In contrast to cell lines from body cavity-based lymphomas derived from HIV-positive individuals that contain both KSHV and Epstein Barr viral genome, these cells contain only KSHV, allowing for uncontaminated virologic studies. Ultrastructural examination identified malignant cells with features of late differentiating B cells (immunoblasts). Cells with viral cytopathic effect contained typical 110-nm intranuclear herpesvirus nucleocapsids and complete cytoplasmic virions, confirming the association of PEL with KSHV. (C) 1996 by The American Society of Hematology.