STRUCTURE AND EXPRESSION OF THE HAIRLESS GENE OF MICE
STRUCTURE AND EXPRESSION OF THE HAIRLESS GENE OF MICE
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DOI：
10.1073/pnas.91.16.7717
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发表时间：
1994-08-02
影响因子：
11.1
通讯作者：
BEST, S
中科院分区：
文献类型：
--
作者：
CACHONGONZALEZ, MB;FENNER, S;BEST, S
小鼠的无毛突变是由小鼠白血病病毒插入引起的。从前病毒侧翼的序列开始，获得围绕病毒整合位点的一系列重叠克隆。通过测序、PCR和外显子捕获技术的组合，鉴定了无毛基因。它编码一个1182个氨基酸的蛋白质，包括一个潜在的锌指结构域。该基因的表达模式密切反映了携带无毛突变的动物的表型。
The hairless mutation of mice was caused by insertion of a murine leukemia virus. Starting with sequences flanking the provirus, a series of overlapping clones surrounding the viral integration site were obtained. By using a combination of sequencing, PCR, and exon-trapping techniques, the hairless gene was identified. It encodes a predicted protein of 1182 amino acids, including a potential zinc finger domain. The expression patterns of the gene closely reflect the phenotype of animals carrying the hairless mutation.