Detection and Phylogenetic Analysis of Peste des Petits Ruminants Virus Isolated from Outbreaks in Punjab, Pakistan
Detection and Phylogenetic Analysis of Peste des Petits Ruminants Virus Isolated from Outbreaks in Punjab, Pakistan
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DOI：
10.1111/j.1865-1682.2011.01245.x
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发表时间：
2012-02-01
影响因子：
4.3
通讯作者：
Berg, M.
中科院分区：
文献类型：
--
作者：
Munir, M.;Zohari, S.;Berg, M.
小反刍兽疫（Peste des Petits Ruminants， PPR）是一种重要的小反刍动物病毒性疾病，在巴基斯坦流行。在接下来的研究中，对山羊中两次小反刍兽疫暴发的样本进行了实验室调查。采用常规PCR和实时PCR对小反刍兽疫病毒（PPRV）基因组进行了检测。通过对PPRV融合基因(F)和核蛋白(N)基因（255 bp）的测序，对当地PPRV野区分离株进行遗传鉴定。基于F基因的系统发育树将来自两次暴发的样本与其他亚洲分离株聚集在谱系4中，特别是与来自中东的分离株聚集在亚簇1中。对N基因的分析显示出不同的模式。在这种情况下，巴基斯坦的样本与中国、塔吉克斯坦和伊朗的分离株聚集在一起，这可能代表了病毒传播的真实地理格局。这是首次提出基于N基因的系统发育树，并对巴基斯坦分离株的F和N基因树进行平行比较。本研究的结果揭示了巴基斯坦PPRV种群，并强调了使用分子方法了解流行病学的重要性。这种理解对于控制巴基斯坦等流行国家发生的小反刍兽疫暴发的数量和影响的任何努力都是至关重要的，特别是在目前的情况下，世界动物卫生组织和粮农组织迫切希望控制并随后从全球根除小反刍兽疫，正如牛瘟所取得的成就。
Peste des Petits Ruminants (PPR) is an important viral disease of small ruminants and is endemic in Pakistan. In the following study, samples from two outbreaks of PPR in goats have been subjected to laboratory investigations. The Peste des Petits Ruminants virus (PPRV) genome was detected using both conventional and real-time PCR. Genetic characterization of the local PPRV field isolates was conducted by sequencing 322 bp of the fusion (F) gene and 255 bp of the nucleoprotein (N) gene. The phylogenetic tree based on the F gene clustered samples from both outbreaks into lineage 4 along with other Asian isolates, specifically into subcluster 1 along with isolates from Middle East. Analysis of N gene revealed a different pattern. In this case, the Pakistani samples clustered with Chinese, Tajikistani and Iranian isolates, which probably represents the true geographical pattern of virus circulation. This is the first report presenting the phylogenetic tree based on N gene as well as performing a parallel comparison of the trees of F and N gene together from Pakistani isolates. The results of this study shed light on the PPRV population in Pakistan and emphasize the importance of using molecular methods to understand the epidemiology. Such understanding is essential in any efforts to control the number and impact of outbreaks that are occurring in endemic countries such as Pakistan, especially in the current scenario where OIE and FAO are eager to control and subsequently eradicate PPR from the globe, as has been achieved for Rinderpest.