ms3 eliminates ratio distortion in isobaric multiplexed quantitative

ms3 eliminates ratio distortion in isobaric multiplexed quantitative
复制标题

DOI：
--
复制
发表时间：
2011
期刊：
--
影响因子：
--
通讯作者：
R. Rad;S. Gygi;W. Haas
R. Rad;S. Gygi;W. Haas
中科院分区：
其他
文献类型：
--
作者：
R. Rad;S. Gygi;W. Haas

文献摘要

被引文献

相似文献

的双蛋白质组模型样本，以准确地测量从大规模蛋白质组学实验的定量数据的干扰效应的程度。我们使用这个模型来评估几种分析策略作为对概述的困难的潜在解决方案，并且我们表明执行额外的分离和片段化事件（MS3扫描）通过消除干扰效应克服了这些问题。
of a two-proteome model sample to accurately measure the extent of the interference effect on quantitative data from a large-scale proteomics experiment. We used this model to evaluate several analytical strategies as potential solutions to the outlined diffi-culties, and we show that performing an additional isolation and fragmentation event (MS3 scan) overcomes these problems by eliminating the interference effect.We used sixplexed TMT reagents to build a model two- proteome peptide mixture sample from Lys-C protein digests of yeast (