Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation.
Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation.
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细胞松弛素 B 和放线菌酮对孤雌刺激后猪卵母细胞活化率、染色体组成和体外发育的影响。
DOI：
10.1071/r96078
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
K. Chung
中科院分区：
文献类型：
--
作者：
S. K. Cha;N. Kim;S. M. Lee;C. S. Baik;H. T. Lee;K. Chung
在孤雌生殖刺激后，在存在或不存在细胞松弛素 B 和放线菌酮的情况下，检查了猪卵母细胞的激活率、染色体组成和发育模式。乙醇或 Ca2+ 离子载体处理后用放线菌酮处理增加了活化的发生率。用细胞松弛素 B 处理的卵母细胞中具有两个或多个雌性原核的卵母细胞的百分比高于对照或放线菌酮处理的卵母细胞（P < 0.05）。与对照组和放线菌酮处理组相比，电刺激和细胞松弛素 B 处理增加了二倍体染色体扩散的发生率，并加速了桑椹胚和囊胚阶段的发育，表明倍性在单性动物发育中的作用。
Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P < 0.05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote.