Activation of complement by cytoskeletal intermediate filaments
Activation of complement by cytoskeletal intermediate filaments
复制标题
细胞骨架中间丝激活补体
作者：
E. Linder;V. Lehto;S. Stenman
自从细胞胞质中间（10纳米）纤维从微管和微丝中成功地进行免疫组织化学分化以来，人们对这些纤维的兴趣越来越大1,2。尽管中间丝在各种细胞类型中广泛存在，但对其功能知之甚少1,3,4。基于它们在细胞内的分布，它们与质膜和细胞核的连接，以及它们在洗涤剂和盐溶液中的显著不溶性，已经提出了细胞骨架核锚定作用5,6。患者血清中存在针对中间纤维的自身抗体7,8，表明这些纤维可能参与了发病过程。我们在此报道，在涉及中间丝的可能的发病机制的研究中，已经观察到补体成分与中间丝的抗体非依赖性结合。最初的观察是用培养的人类胚胎成纤维细胞进行的，因为这些细胞中的细胞质丝已经通过使用免疫标记物和能够选择性改变微丝、中间丝和微管形态的药物进行了表征。随后的研究表明，补体与其他培养细胞和组织切片细胞的类似细胞质结构结合。
INTEREST in cytoplasmic intermediate (10 nm) filaments has grown since the successful immunohistochemical differentiation of these filaments from microtubules and microfilaments1,2. Despite the widespread occurrence of intermediate filaments in various cell types, little is known about their function1,3,4. A cytoskeletal nucleus-anchoring role has been suggested based on their intracellular distribution, their connections to the Plasma membrane and nucleus, and their marked insolubility in detergents and salt solutions5,6. The presence of autoantibodies against intermediate filaments in the sera of patients7,8 suggests that these filaments can be involved in pathogenetic processes. We report here that in studies on possible pathogenetic mechanisms involving intermediate filaments, antibody-independent binding of complement components to intermediate filaments has been observed. Initial observations were made using cultured human embryonal fibroblasts, as cytoplasmic filaments present in these cells have been characterised by using both immunological markers and drugs capable of selectively altering the morphology of microfilaments, intermediate filaments and microtubules1,7. Subsequent studies showed complement binding to similar cytoplasmic structures of other cultured cells and also cells in tissue sections.