Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos
Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos
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DOI：
10.1016/j.yexcr.2006.08.013
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发表时间：
2006-11-01
影响因子：
3.7
通讯作者：
Sheng, Hui Z.
中科院分区：
文献类型：
--
作者：
Fang, Zhen F.;Gai, Hui;Sheng, Hui Z.
从受精（传统 rbES 细胞）、孤雌生殖（pES 细胞）和核移植（ntES 细胞）产生的兔囊胚中分离胚胎干细胞，并在无血清培养系统中增殖。兔ES（rbES）细胞在未分化状态下长时间增殖并保持正常核型。这些细胞以单层形式生长，具有高核/细胞质比，并含有高水平的碱性磷酸盐活性。此外，rbES细胞表达多能标记Oct-4以及EBAF2、FGF4、TDGF1，但不表达针对SSEA-1、SSEA-3、SSEA-4、TRA-1-10和TRA-1-81的抗体识别的抗原。所有 3 种类型的 ES 细胞均形成拟胚体并产生包含所有三种胚层组织类型的畸胎瘤。 rbES细胞表现出高克隆效率，易于进行基因改造，并可用作核供体，通过体细胞核移植产生可存活的兔子。与基因工程相结合，胚胎干细胞技术将有助于培育新的兔系。 (c) 2006 Elsevier Inc. 保留所有权利。
Embryonic stem cells were isolated from rabbit blastocysts derived from fertilization (conventional rbES cells), parthenogenesis (pES cells) and nuclear transfer (ntES cells), and propagated in a serum-free culture system. Rabbit ES (rbES) cells proliferated for a prolonged time in an undifferentiated state and maintained a normal karyotype. These cells grew in a monolayer with a high nuclear/cytoplasm ratio and contained a high level of alkaline phosphate activity. In addition, rbES cells expressed the pluripotent marker Oct-4, as well as EBAF2, FGF4, TDGF1, but not antigens recognized by antibodies against SSEA-1, SSEA-3, SSEA-4, TRA-1-10 and TRA-1-81. All 3 types of ES cells formed embryoid bodies and generated teratoma that contained tissue types of all three germ layers. rbES cells exhibited a high cloning efficiency, were genetically modified readily and were used as nuclear donors to generate a viable rabbit through somatic cell nuclear transfer. in combination with genetic engineering, the ES cell technology should facilitate the creation of new rabbit lines. (c) 2006 Elsevier Inc. All rights reserved.