A gene expression vector useful for protein purification and studies of protein-protein interaction.
A gene expression vector useful for protein purification and studies of protein-protein interaction.
复制标题
一种可用于蛋白质纯化和蛋白质-蛋白质相互作用研究的基因表达载体。
DOI：
10.1016/0378-1119(93)90032-x
复制
发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Narayan,O
中科院分区：
文献类型：
--
作者：
Zhao,LJ;Narayan,O
本报告描述了一种基因表达载体的开发，该载体在假定合成的蛋白质的c端增加了三个特征：(1)一个蛋白激酶a识别域，允许蛋白质在体外进行放射标记，(2)一个用商业单克隆抗体对蛋白质进行免疫表征的表位标记，(3)一个（His）6块，便于通过金属螯合亲和层析纯化蛋白质。这些特征使得对感兴趣的蛋白质进行生化和功能分析变得方便。
This report describes the development of a gene expression vector that adds three features to the C-terminus of the putative synthesized protein: (1) a protein kinase A recognition domain allowing the protein to be radio-labeled in vitro, (2) an epitope marker for immunocharacterization of the protein with a commercial monoclonal antibody, and (3) a (His)6block facilitating purification of the protein by metal chelating affinity chromatography. These features make it convenient to perform biochemical and functional analysis of the protein of interest.