Emerging tools for real-time label-free detection of interactions on functional protein microarrays
Emerging tools for real-time label-free detection of interactions on functional protein microarrays
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DOI：
10.1111/j.1742-4658.2005.04971.x
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发表时间：
2005-11-01
期刊：
影响因子：
5.4
通讯作者：
LaBaer, J
中科院分区：
文献类型：
--
作者：
Ramachandran, N;Larson, DN;LaBaer, J
广泛的基因组信息和内容的可用性已经催生了一个高通量筛选的时代，该时代正在产生大量的功能基因组数据。特别是，需要了解细胞内的生物化学布线引入了新的方法来映射蛋白质相互作用产生的复杂的生物相互作用网络。目前的蛋白质相互作用的检测技术-酵母双杂交和免疫沉淀与质谱检测-已经取得了相当大的成功。然而，这些方法的并行使用仅确定了蛋白质之间生理相关相互作用的一小部分，忽略了所有非蛋白质相互作用，例如与代谢物、脂质、DNA和小分子的相互作用。这突出了进一步开发蛋白质组规模技术的必要性，从而能够研究蛋白质功能。在这里，我们讨论了在功能蛋白质微阵列上显示蛋白质的高通量技术的最新进展，以及使用局部折射率探针、碳纳米管和纳米线或微机电系统微杠杆实时无标记检测相互作用。这些技术的结合将促进蛋白质与蛋白质以及与其他生物分子相互作用的大规模研究。
The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of functional genomic data. In particular, the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins. The current technologies for assaying protein interactions - yeast two-hybrid and immunoprecipitation with mass spectrometric detection - have met with considerable success. However, the parallel use of these approaches has identified only a small fraction of physiologically relevant interactions among proteins, neglecting all nonprotein interactions, such as with metabolites, lipids, DNA and small molecules. This highlights the need for further development of proteome scale technologies that enable the study of protein function. Here we discuss recent advances in high-throughput technologies for displaying proteins on functional protein microarrays and the real-time label-free detection of interactions using probes of the local index of refraction, carbon nanotubes and nanowires, or microelectromechanical systems cantilevers. The combination of these technologies will facilitate the large-scale study of protein interactions with proteins as well as with other biomolecules.