CXCR4 enhances cisplatin resistance of human tongue squamous cell carcinoma
CXCR4 enhances cisplatin resistance of human tongue squamous cell carcinoma
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CXCR4增强人舌鳞状细胞癌的顺铂耐药性
DOI：
10.1111/jop.12813
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Zhou Bin
中科院分区：
文献类型：
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作者：
Zhuang Xiu-mei;Zhou Bin
背景：趋化因子受体4 （CXCR4）在肿瘤进展中起重要作用。CXCR4过表达与患者预后不良相关。头颈部鳞状细胞癌。然而，两者之间的相关性。舌鳞癌（TSCC）中CXCR4与化疗耐药的关系。方法：建立稳定的顺铂耐药CAL27 CDDP和SCC25 CDDP细胞，用CCK8法鉴定，并检测CXCR4的表达。采用qRT‐PCR和Western blot检测。将CXCR4‐siRNA转染到TSCC CDDP中。细胞，检测转染效率。结果：成功建立了CAL27 CDDP和SCC25 CDDP细胞。在顺铂刺激下，细胞活力明显高于亲本细胞，细胞凋亡明显减少。CXCR4在TSCC cddp细胞中表达升高。转染CXCR4 - siRNA后，CXCR4的表达降低。在CAL27 CDDP和SCC25 CDDP细胞中分别为73%和78%。CCK8化验。流式细胞术检测显示顺铂作用下细胞的增殖能力。CXCR4沉默后刺激显著减少。此外，TSCC升高。敲除CXCR4后，CDDP细胞停留在G0/G1期。与阴性对照组相比，cyclin D1和p‐akt .的表达降低，p‐caspase‐3和Bax的表达显著升高。结论：沉默CXCR4可明显抑制细胞增殖，诱导细胞凋亡。并通过降低细胞周期蛋白D1和p‐来增强TSCC CDDP细胞的顺铂敏感性。p - caspase - 3和Bax均升高。
Background: The chemokine receptor 4 (CXCR4) plays an important role in tumor.progression. Overexpressed CXCR4 is associated with a poor prognosis of patient.with head and neck squamous cell carcinomas. However, the correlation between.CXCR4 and chemotherapy resistance in tongue squamous cell carcinoma (TSCC).remains obscure..Methods: Stable cisplatin‐resistant CAL27 CDDP and SCC25 CDDP cells were.established and identified by CCK8 assay, and the CXCR4 expression was detected.using qRT‐PCR and Western blot. CXCR4‐siRNA was transfected into TSCC CDDP.cells, whose transfect efficiency was examined. Cisplatin sensitivity was further.detected, as well as several proliferation and apoptosis‐related proteins..Results: CAL27 CDDP and SCC25 CDDP cells were successfully established, which.exhibited significantly higher cell viability and less apoptosis under cisplatin stimulation than that of parental cells. CXCR4 expression was increased in TSCC CDDP.cells. After transfection of CXCR4‐siRNA, the expression of CXCR4 was reduced by.73% and 78% in CAL27 CDDP and SCC25 CDDP cells, respectively. CCK8 assay.and flow cytometry assay revealed that the proliferative capacity under cisplatin.stimulation significantly decreased after CXCR4 silencing. Moreover, increased TSCC.CDDP cells were arrested in the G0/G1 phase after knockdown of CXCR4..Compared with negative control group, the expression of cyclin D1 and p‐AKT.decreased, while that of p‐caspase‐3 and Bax significantly increased..Conclusions: Silencing CXCR4 may evidently inhibit proliferation, induce apoptosis.and enhance cisplatin sensitivity of TSCC CDDP cells by reduced cyclin D1 and p‐.AKT, and increased p‐caspase‐3 and Bax.