High Levels of Interferon-Alpha Expressing Macrophages in Human Breast Milk During SARS-CoV-2 Infection: A Case Report.
High Levels of Interferon-Alpha Expressing Macrophages in Human Breast Milk During SARS-CoV-2 Infection: A Case Report.
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SARS-CoV-2 感染期间人母乳中表达高水平干扰素-α 的巨噬细胞：病例报告。
DOI：
10.1089/bfm.2020.0369
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Baban,Babak
中科院分区：
文献类型：
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作者：
Yu,JackC;Khodadadi,Hesam;Salles,ÉvilaLopes;Pham,Quyen;Patel,Pinkal;Baban,Babak
引言：除了洗手和戴口罩外，保持社交距离和将接触时间减少到15分钟以下是防止COVID-19传播的最有效措施。不幸的是，其中三个准则对于哺乳婴儿来说非常困难，如果不是不可能的话：他们不能戴口罩，不能离开哺乳乳房六英尺，也不能在哺乳期间在15分钟内完成。我们报告的情况下，与SARS-CoV-2感染的哺乳期的母亲，记录的变化，免疫细胞和细胞因子在母乳中的感染和没有infection.Case描述：与机构审查委员会（IRB）的批准，我们获得了表达的母乳样本从哺乳期的母亲之前和期间的SARS-CoV-2感染记录的逆转录-PCR。采用流式细胞仪分析，我们测量了免疫细胞谱和细胞因子如干扰素α（IFNα）在感染前和感染过程中的牛奶白细胞中的表达。结果：IFNα+牛奶白细胞增加了8倍，从SARS-CoV-2感染前的1%增加到活动性感染时的8%。乳巨噬细胞显示IFNα表达的最高增加。T、B淋巴细胞均轻度升高。先天淋巴细胞、中性粒细胞和自然杀伤细胞的IFNα表达没有增加，而树突状细胞实际上表现出减少。结论：我们记录了确诊为COVID-19的哺乳期母亲的母乳巨噬细胞中存在IFNα且表达较高，与感染前的乳汁相比。
Introduction:In addition to hand washing and wearing masks, social distancing and reducing exposure time to <15 minutes are the most effective measures against the spread of COVID-19. Unfortunately, three of these guidelines are very difficult, if not impossible, for nursing babies: they cannot wear masks, stay six feet away from the lactating breasts, nor consistently finish within 15 minutes while nursing. We report a case of a nursing mother with SARS-CoV-2 infection, documenting changes of immune cells and cytokines in breast milk with and without the infection.Case Description:With Institutional Review Board (IRB) approval, we obtained expressed breast milk samples from a lactating mother before and during SARS-CoV-2 infection as documented by reverse transcription-PCR. Using flow cytometry analysis, we measured the immune cell profiles and expression of cytokines such as interferon alpha (IFNα) in milk leukocytes before and during infection.Results:There was an eightfold increase in IFNα+ milk leukocytes, from 1% before SARS-CoV-2 infection to 8% when actively infected. The milk macrophages showed the highest increase in IFNα expression. Both T and B lymphocytes showed mild increase. Innate lymphoid cells, neutrophils, and natural killer cells showed no increase in IFNα expression and the dendritic cells actually showed a reduction.Conclusion:We document the presence and high expression of IFNα in the breast milk macrophages of a lactating mother with confirmed COVID-19, compared with her milk before the infection.