Mesenchymal stem cells can be obtained from the human saphena vein
Mesenchymal stem cells can be obtained from the human saphena vein
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DOI：
10.1016/j.yexcr.2005.06.005
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发表时间：
2005-10-01
影响因子：
3.7
通讯作者：
Zago, MA
中科院分区：
文献类型：
--
作者：
Covas, DT;Piccinato, CE;Zago, MA
间充质干细胞(MSC)可以从成人和胎儿的许多部位分离出来。目前已从牛动脉壁分离出具有成骨、软骨、成骨和基质形成能力的细胞，我们现在证明也可以从成人的静脉壁中分离出MSC。从隐静脉内表面分离出来的细胞在体外培养2-3周，每周复制一次。体外培养形成半融合层的梭形细胞CD13+、CD29+、CD44+、CD34-、CD45-、CD14-、CD133-、CD31-、CD33-、CD54+、CD106-、CD90+、KDR-、钙粘蛋白-5-、人类白细胞抗原I类和人类白细胞抗原DR-，并在体外向成骨细胞、软骨细胞和脂肪细胞分化。与其他七种正常组织相比，基因表达与从其他来源获得的骨髓间充质干细胞有很强的相似性。有三个基因在隐匿性MSC中的表达高于其他两个MSC，其中两个基因的表达与血管生成有关，其中两个基因的表达与血管内皮细胞共享。这些结果表明，人静脉壁含有间充质细胞，其形态特征、免疫表型标记、基因表达谱和分化潜能与从骨髓和脐静脉获得的间充质干细胞相似。(C)2005 Elsevier Inc.保留所有权利。
Mesenchymal stem cells (MSC) can be isolated from many sites adults and the fetus. Cells with osteoblastic, chondrogenic, leiomiogenic and stromogenic potentials have been obtained from the bovine artery wall, and we now show that MSC can be isolated also from the adult human vein wall. Cells detached from internal surface of the saphenous vein are cultured in vitro for 2-3 weeks and replated weekly. The culture forms a semi-confluent layer of spindle-shaped cells that are CD13+, CD29+, CD44+, CD34-, CD45-, CD14-, CD133-, CD31-, CD33-, CD54+, CD106-, CD90+, KDR-, cadherin-5-, HLA class I+ and HLA-DR- and differentiate in vitro into osteoblasts, chondrocytes and adipocytes. Gene expression, when compared with seven other normal tissues, shows strong similarity with MSC obtained from other sources. Three genes more expressed in saphenous MSC than in the other two MSC are related to angiogenesis, and the expression of two of them is shared by endothelial cells. These results demonstrate that the human vein wall contains mesenchymal cells with morphologic features, immunophenotypic markers, gene expression profile and differentiation potential that are similar to MSC obtained from the bone marrow and from the umbilical vein. (c) 2005 Elsevier Inc. All rights reserved.