In vivo evaluation of porous lithium-doped hydroxyapatite scaffolds for the treatment of bone defect
In vivo evaluation of porous lithium-doped hydroxyapatite scaffolds for the treatment of bone defect
复制标题
多孔锂掺杂羟基磷灰石支架治疗骨缺损的体内评价。
DOI：
10.3233/bme-181018
复制
发表时间：
2018-01-01
影响因子：
1
通讯作者：
Kang, PengDe
中科院分区：
文献类型：
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作者：
Luo, Yue;Li, Donghai;Kang, PengDe
羟基磷灰石(HA)具有与骨相似的矿物成分，具有良好的物理化学性质。单纯的羟基磷灰石支架虽然脆性较大，但在促进血管生成和诱导成骨方面存在不足。本研究旨在评估锂(Li)掺入羟基磷灰石(HA)能否改善支架的固有缺陷。本实验对锂-羟基磷灰石支架的机械强度、生物相容性和生物降解性进行了初步研究。在体内研究中，将锂羟基磷灰石支架植入动物模型中修复骨缺损。同时检测血管生成因子和成骨因子的表达。作为对照，设计了自体骨、羟基磷灰石和空白对照组。结果表明，锂与羟基磷灰石的复合对支架的降解速度没有显著影响，但获得了较高的压缩机械强度。掺杂Li后，骨再生进一步增强，但血管生成效果不明显。体内实验表明，Li-HA支架可促进GSK-3？-连环蛋白降低，-连环蛋白升高。综上所述，在羟基磷灰石中掺杂Li是改善羟基磷灰石的力学性能和促进其成骨潜能的一种替代策略。仅根据这项研究，强烈建议将该方法应用于临床。
Hydroxyapatite (HA) possesses similar mineral components to bone and possesses good physicochemical properties. Even though pure HA scaffold is brittle, it is insufficient in promoting vascularization and osteoinductivity. This study was conducted to assess whether lithium (Li) incorporated into HA could improve the scaffolds' inherent shortcomings. In the experiments, Li-hydroxyapatite scaffolds' mechanical strength, biocompatibility, and biodegradability were researched primarily. In vivo studies, the Li hydroxyapatite scaffolds were implanted into an animal model to repair the bone defects. Meanwhile, we also evaluated the expression of angiogenic and osteogenic factors. For comparison, autologous bone, hydroxyapatite, and blank control groups were designed. According to the results, Li incorporated with hydroxyapatite did not significantly change the scaffold's degradation velocity, but it obtained higher compress mechanical strength. After Li was doped, bone regeneration was further enhanced but the angiogenic effect was not improved significantly. The in vivo study, Li-HA scaffolds improved new bone formation with GSK-3? decreased and ?-catenin increased. In conclusion, doped Li into hydroxyapatite was an alternative strategy for improving hydroxyapatite's mechanical property and promoting the osteogenesis potential. This method is highly recommended for clinical application based on this study alone.