Morphology of the olivocerebellar projection of the chick: An axonal reconstruction study.
Morphology of the olivocerebellar projection of the chick: An axonal reconstruction study.
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雏鸡橄榄小脑投射的形态学：轴突重建研究。
DOI：
10.1002/cne.23352
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
I.
中科院分区：
文献类型：
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作者：
Sasanuma;K.;Ohki-Hamazaki;H. and Sugihara;I.
橄榄小脑(OC)轴突的投射模式紧密地反映了大鼠小脑的区划和发育组织，其终末主要为小脑皮质中的爬升纤维(CFs)。鸟类的小脑与哺乳动物的小脑一样，具有良好的分叶和纵向分隔。然而，在鸟类小脑的大部分区域，OC轴突的投射模式还没有得到详细的研究。在本研究中，我们重建了通过向下橄榄内注射生物素化葡聚糖胺而产生的标记的鸡OC轴突，以研究它们的形态特征，并确定它们与鸡小脑的一般形态的关系。标记的CFs基本上分布在小叶I-VIII的单个纵向延长的窄带状区域，但在小叶IX-X分布于多个横向加宽的带状区域。四个重建的OC轴突中有三个终止于小叶IXa-c的单一纵向带状区域，另一个终止于小叶IXc的多个内侧分离的区域，是小叶的一部分。单个OC轴突平均分出14个CFs。两个CFs偶尔合并成一个单一的末端乔木。轴突也有细小的非CF侧支投射到小脑核或皮质。结果表明，鸡和大鼠OC轴突的形态特征，包括分支和终末，基本保守。J.Comp.神经。2013年521：3321-3339。©2013 Wiley期刊，Inc.
The projection pattern of the olivocerebellar (OC) axons, which terminate mainly as climbing fibers (CFs) in the cerebellar cortex, tightly reflects the compartmental and developmental organization of the cerebellum as revealed by mapping and reconstruction studies in the rat. The avian cerebellum is well lobulated and longitudinally compartmentalized like the mammalian cerebellum. However, the projection pattern of the OC axons has not been studied in detail for most areas of the avian cerebellum. In the present study, we reconstructed labeled chick OC axons resulting from a small focal injection of biotinylated dextran amine into the inferior olive to investigate their morphological characteristics, and to determine their relationship to the general morphology of the chick cerebellum. Labeled CFs were distributed basically in a single longitudinally elongated narrow band‐shaped area in lobules I–VIII, but in multiple, transversely widened, band‐shaped areas in lobules IX–X. Three of the four reconstructed OC axons terminated in a single longitudinally band‐shaped area in lobules IXa–c, whereas the other one terminated in multiple mediolaterally separated areas in lobule IXc, which is part of the flocculus. Single OC axons branched into 14 CFs on average. Two CFs occasionally merged to form a single terminal arbor. Axons also had thin, non‐CF collaterals that projected either to a cerebellar nucleus or to the cortex. The results indicate that the morphological characteristics of OC axons, including branching and termination, are basically conserved between the chick and the rat. J. Comp. Neurol. 521:3321–3339, 2013. © 2013 Wiley Periodicals, Inc.