Raman spectroscopic signature of life in a living yeast cell
Raman spectroscopic signature of life in a living yeast cell
复制标题
DOI：
10.1002/jrs.1219
复制
发表时间：
2004-07-01
影响因子：
2.5
通讯作者：
Hamaguchi, H
中科院分区：
文献类型：
--
作者：
Huang, YS;Karashima, T;Hamaguchi, H
我们已经发现了一个拉曼光谱特征，它清晰地反映了活酵母细胞中一个小分子的代谢活性。在GFP标记的酵母细胞上的拉曼映射实验表明，该特征完全来自线粒体。加入KCN后，磷脂拉曼谱带迅速减少，随后消失，表明KCN首先抑制了呼吸作用，然后降低了代谢活性，逐渐破坏了磷脂的双膜结构。我们现在可以通过时间和空间分辨的拉曼光谱来监测单个细胞的生与死。版权所有（C）2004约翰威利父子有限公司。
We have discovered a Raman spectroscopic signature that sharply reflects the metabolic activity of a mitochondrion in a living yeast cell. Raman mapping experiments on a GFP-labeled yeast cell showed that this signature originated exclusively from mitochondria. Addition of KCN caused a rapid decrease and subsequent disappearance of the signature followed by gradual changes of the phospholipid Raman bands, indicating that respiration was first inhibited by KCN and then lowered metabolic activity gradually deteriorated the double-membrane structure of a mitochondrion. We can now monitor the life and death of a single cell by time- and space-resolved Raman spectroscopy. Copyright (C) 2004 John Wiley Sons, Ltd.