Hydrogen peroxide-mediated protein oxidation in young and old human MRC-5 fibroblasts
Hydrogen peroxide-mediated protein oxidation in young and old human MRC-5 fibroblasts
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DOI：
10.1006/abbi.1999.1657
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发表时间：
2000-03-01
影响因子：
3.9
通讯作者：
Grune, T
中科院分区：
文献类型：
--
作者：
Merker, K;Sitte, N;Grune, T
提示衰老过程依赖于自由基的作用。细胞代谢的年龄相关变化的亮点之一是氧化蛋白的积累。本研究旨在揭示急性氧化应激过程中和应激后蛋白质氧化和蛋白酶体活性的增殖相关变化。可以证明，细胞溶质蛋白酶体系统的活性在人MRC-5成纤维细胞的增殖衰老期间下降，并且不能有效地去除老细胞中的氧化蛋白。然而，在年轻细胞中，氧化蛋白的去除伴随着整体蛋白质周转的增加，在老年MRC-5成纤维细胞中看不到这种蛋白质周转的增加。因此，我们的研究表明，老年成纤维细胞在氧化应激后更容易受到氧化蛋白积累的影响，并且不能像年轻成纤维细胞那样有效地去除这些氧化蛋白。(C)北京大学出版社.
It is suggested that the aging process is dependent on the action of free radicals. One of the highlights of age-related changes of cellular metabolism is the accumulation of oxidized proteins. The present investigation was undertaken to reveal the proliferation-related changes in the protein oxidation and proteasome activity during and after an acute oxidative stress. It could be demonstrated that the activity of the cytosolic proteasomal system declines during proliferative senescence of human MRC-5 fibroblasts and is not able to remove oxidized proteins in old cells efficiently. Whereas in young cells removal of oxidized proteins was accompanied by an increase in the overall protein turnover, this increase in protein turnover could not be seen in old MRC-5 fibroblasts, Therefore, our studies demonstrate that old fibroblasts ape much more vulnerable to the accumulation of oxidized proteins after oxidative stress and are not able to remove these oxidized proteins as efficiently as young fibroblasts. (C) 2000 Academic Press.